Anti-Mitochondrial Pyruvate dehydrogenase kinase 1抗体[4A11] (ab110025)

概述

  • 产品名称Anti-Mitochondrial Pyruvate dehydrogenase kinase 1抗体[4A11]
    参阅全部 Mitochondrial Pyruvate dehydrogenase kinase 1 一抗
  • 描述
    小鼠单克隆抗体[4A11] to Mitochondrial Pyruvate dehydrogenase kinase 1
  • 经测试应用适用于: WB, ICC/IF, IHC-P, ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Monkey
  • 免疫原

    Recombinant fragment corresponding to Human Mitochondrial Pyruvate dehydrogenase kinase 1.

  • 阳性对照
    • NIH3T3, Hela, Jurkat, HepG2, PC-12, and Cos7 cell lysate, human breast cancer and Human brain tissues, HELA and Lovo cells

应用

Our Abpromise guarantee covers the use of ab110025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Detects a band of approximately 44 kDa (predicted molecular weight: 49 kDa).
ICC/IF 1/200 - 1/1000.
IHC-P 1/200 - 1/1000.
ELISA 1/10000.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [4A11] 图像

  • All lanes : Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [4A11] (ab110025) at 1/500 dilution

    Lane 1 : NIH3T3 cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : PC-12 cell lysate
    Lane 6 : Cos7 cell lysate


    Predicted band size : 49 kDa
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using ab110025 aty a dilution of 1/200 with DAB staining.
  • Immunohistochemical analysis of paraffin-embedded Human brain tissues using ab110025 aty a dilution of 1/200 with DAB staining.
  • Immunofluorescence analysis of HELA cells using ab110025 at a dilution of 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Flow cytometric analysis of Lovo cells using ab110025 (green) and negative control (purple).

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody [4A11] (ab110025)参考文献

This product has been referenced in:
  • Vavrova E  et al. Muscle expression of a malonyl-CoA-insensitive carnitine palmitoyltransferase-1 protects mice against high-fat/high-sucrose diet-induced insulin resistance. Am J Physiol Endocrinol Metab 311:E649-60 (2016). Read more (PubMed: 27507552) »
  • Wang LY  et al. KDM4A Coactivates E2F1 to Regulate the PDK-Dependent Metabolic Switch between Mitochondrial Oxidation and Glycolysis. Cell Rep 16:3016-27 (2016). Read more (PubMed: 27626669) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - other (muscle and liver)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10%)
Loading amount 30 µg
Specification muscle and liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jun 30 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Mouse Cell (Liver mouse tissue)
Specification Liver mouse tissue
Permeabilization No
Fixative Methanol
Username

Dr. Dimitra Kalamida

Verified customer

提交于 Dec 09 2013

Thank you for contacting us.

As always, if we do not advise a certain method on our datasheet, the customer can use the one which they usually use within their lab. This is the case of ab110025.

For ab65979 I would suggest checking th...

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Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from these antibodies.

The details your customer has kindly provi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"