Anti-PDK1抗体[4A11] (ab110025)
Key features and details
- Mouse monoclonal [4A11] to PDK1
- Suitable for: WB, ICC/IF, IHC-P, ELISA, Flow Cyt
- Reacts with: Mouse, Rat, Human, Monkey, African green monkey
- Isotype: IgG1
概述
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产品名称
Anti-PDK1抗体[4A11]
参阅全部 PDK1 一抗 -
描述
小鼠单克隆抗体[4A11] to PDK1 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, IHC-P, ELISA, Flow Cytmore details -
种属反应性
与反应: Mouse, Rat, Human, Monkey, African green monkey -
免疫原
Recombinant fragment corresponding to Human PDK1 aa 308-436.
Database link: Q15118 -
阳性对照
- Hela, Jurkat, HepG2, PC-12, and Cos7 cell lysate, human breast cancer and Human brain tissues, HELA and Lovo cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
4A11 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110025于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/500 - 1/2000. Detects a band of approximately 44 kDa (predicted molecular weight: 49 kDa).
|
ICC/IF | (1) |
1/200 - 1/1000.
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IHC-P |
1/200 - 1/1000.
|
|
ELISA |
Use at an assay dependent concentration.
|
|
Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/500 - 1/2000. Detects a band of approximately 44 kDa (predicted molecular weight: 49 kDa). |
ICC/IF
1/200 - 1/1000. |
IHC-P
1/200 - 1/1000. |
ELISA
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. -
组织特异性
Expressed predominantly in the heart. -
序列相似性
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 5163 Human
- Entrez Gene: 228026 Mouse
- Entrez Gene: 116551 Rat
- Omim: 602524 Human
- SwissProt: Q15118 Human
- SwissProt: Q8BFP9 Mouse
- SwissProt: Q63065 Rat
- Unigene: 470633 Human
see all -
别名
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody
- HGNC:8809 antibody
- Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody
see all
图片
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Lanes 1-2 : Anti-PDK1 antibody [4A11] (ab110025) at 1/500 dilution
Lane 3 : Beta-actin antibody at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : NIH3T3 cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : HRP-Goat Anti-Mouse IgG (Fc) at 1/10000 dilution
Predicted band size: 49 kDa12% SDS gel.
Block the membrane for 1 h at room temperature or overnight at 4°C using blocking buffer, 5% milk.
Incubate in ab110025 overnight at 4°C.
Incubation at room temperature for 1 hour with the secondary antibody.
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All lanes : Anti-PDK1 antibody [4A11] (ab110025) at 1/500 dilution
Lane 1 : NIH3T3 cell lysate
Lane 2 : Hela cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : PC-12 cell lysate
Lane 6 : Cos7 cell lysate
Predicted band size: 49 kDa -
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using ab110025 aty a dilution of 1/200 with DAB staining.
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Immunohistochemical analysis of paraffin-embedded Human brain tissues using ab110025 aty a dilution of 1/200 with DAB staining.
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Immunofluorescence analysis of HELA cells using ab110025 at a dilution of 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Flow cytometric analysis of Lovo cells using ab110025 (green) and negative control (purple).
实验方案
数据表及文件
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Datasheet download
文献 (34)
ab110025 被引用在 34 文献中.
- Goyal M et al. Blood progenitor redox homeostasis through olfaction-derived systemic GABA in hematopoietic growth control in Drosophila. Development 149:N/A (2022). PubMed: 34850846
- Cenigaonandia-Campillo A et al. Vitamin C activates pyruvate dehydrogenase (PDH) targeting the mitochondrial tricarboxylic acid (TCA) cycle in hypoxic KRAS mutant colon cancer. Theranostics 11:3595-3606 (2021). PubMed: 33664850
- Yao S et al. The oncogenic and prognostic role of PDK1 in the progression and metastasis of ovarian cancer. J Cancer 12:630-643 (2021). PubMed: 33403023
- Lee YG et al. LONP1 and ClpP cooperatively regulate mitochondrial proteostasis for cancer cell survival. Oncogenesis 10:18 (2021). PubMed: 33637676
- Xin W et al. lncRNA LA16c-313D11.11 modulates the development of endometrial cancer by binding to and inhibiting microRNA-205-5p function and indirectly increasing PTEN activity. Int J Oncol 57:355-363 (2020). PubMed: 32319598