Anti-Mitochondrial Ferritin抗体(ab93428)

概述

  • 产品名称
    Anti-Mitochondrial Ferritin抗体
    参阅全部 Mitochondrial Ferritin 一抗
  • 描述
    兔多克隆抗体to Mitochondrial Ferritin
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Mitochondrial Ferritin aa 50-150 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q8N4E7
    (Peptide available as ab93799)

  • 阳性对照
    • This antibody gave a positive signal in both Hela and HEK293 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab93428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250. Detects a band of approximately 22 kDa (predicted molecular weight: 27 kDa).

靶标

  • 功能
    Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.
  • 组织特异性
    Detected in testis and erythroleukemia. Expression is very low or not detectable in brain, colon, heart, kidney, liver, lung, muscle, placental, spleen and small intestine.
  • 序列相似性
    Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • 细胞定位
    Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FerH antibody
    • Ferritin antibody
    • Ferritin H subunit antibody
    • Ferritin, mitochondrial antibody
    • Fth 3 antibody
    • Fth3 antibody
    • FTMT antibody
    • FTMT_HUMAN antibody
    • mitochondrial antibody
    • Mitochondrial ferritin antibody
    • MTF antibody
    • OTTHUMP00000159158 antibody
    see all

图片

  • All lanes : Anti-Mitochondrial Ferritin antibody (ab93428) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 minutesThe band observed at 22 kDa could potentially be a cleaved form of Human Ferritin, mitochondrial precursor due to a 49 amino acid transit peptide (SwissProt), which when cleaved gives an expected Mw of 22 kDa.

实验方案

文献

ab93428 has not yet been referenced specifically in any publications.

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