Anti-Mitochondrial Ferritin抗体(ab66111)

概述

  • 产品名称Anti-Mitochondrial Ferritin抗体
    参阅全部 Mitochondrial Ferritin 一抗
  • 描述
    兔多克隆抗体to Mitochondrial Ferritin
  • 经测试应用适用于: WB, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Cow, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human Mitochondrial Ferritin aa 200 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q8N4E7

  • 阳性对照
    • This antibody gave a positive signal in both human liver and brain tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab66111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 27 kDa).
IHC-Fr 1/100.
ICC/IF 1/100.

靶标

  • 功能Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.
  • 组织特异性Detected in testis and erythroleukemia. Expression is very low or not detectable in brain, colon, heart, kidney, liver, lung, muscle, placental, spleen and small intestine.
  • 序列相似性Belongs to the ferritin family.
    Contains 1 ferritin-like diiron domain.
  • 细胞定位Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FerH antibody
    • Ferritin antibody
    • Ferritin H subunit antibody
    • Ferritin, mitochondrial antibody
    • Fth 3 antibody
    • Fth3 antibody
    • FTMT antibody
    • FTMT_HUMAN antibody
    • mitochondrial antibody
    • Mitochondrial ferritin antibody
    • MTF antibody
    • OTTHUMP00000159158 antibody
    see all

Anti-Mitochondrial Ferritin antibody 图像

  • IHC-Fr image of Ferritin, mitochondrial staining on mouse skeletal muscle using ab66111 (1: 100). The sections were fixed in 4% PFA and the section was permeabilized using 0.1% TritonX in 0.1% PBS. The section was then blocked using 10% conkey serum for 1 hour at 24°C. The primary antibody (1:100) was incubated at 4°C for 24 hours and the secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).

  • ICC/IF image of Ferritin, mitochondrial staining on mouse hippocampal culture using ab66111 (1: 100). The cells were fixed in 4% PFA and the section was permeabilized using 0.1% TritonX in 0.1% PBS. The cells was then blocked using 10% donkey serum for 1 hour at 24°C. The primary antibody (1:100) was incubated at 4°C for 24 hours and the secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).

  • All lanes : Anti-Mitochondrial Ferritin antibody (ab66111) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes
    The band observed at 21 kDa could potentially be a cleaved form of Human Ferritin, mitochondrial precursor due to a 49 amino acid transit peptide (SwissProt), which when cleaved gives an expected Mw of 22 kDa.

Anti-Mitochondrial Ferritin antibody (ab66111)参考文献

ab66111 has not yet been referenced specifically in any publications.

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