Mitochondria分离试剂盒for组织(with Dounce Homogenizer) (ab110169)

概述

  • 产品名称Mitochondria分离试剂盒for组织(with Dounce Homogenizer)
    参阅全部 Mitochondria Isolation 试剂盒
  • 样品类型
    Tissue
  • 检测类型Quantitative
  • 产品概述

    ab110169 (MS851) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.

  • 说明

    Tested tissues: brain, liver and heart

  • 经测试应用适用于: Functional Studiesmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab110169 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent dilution. Mitochondria isolations from hard or soft tissues.

    Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer) 图像

    • Rat liver mitochondria were isolated using Abcam's ab110169/MS851 Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer). 2 µg (lane 1) and 10 µg (lane 2) of mitochondria were tested with Abcam's ab110413/MS604 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail). Mitochondria are enriched and so Western blot band intensity of mitochondrial proteins is greater in these mitochondrial preparations when compared to homogenate at respective amounts, see lane 3 and 4

    实验方案

    Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer) (ab110169)参考文献

    This product has been referenced in:
    • Gu F  et al. Alterations in mitochondrial DNA copy number and the activities of electron transport chain complexes and pyruvate dehydrogenase in the frontal cortex from subjects with autism. Transl Psychiatry 3:e299 (2013). Read more (PubMed: 24002085) »
    • Lehmann HC  et al. Mitochondrial dysfunction in distal axons contributes to human immunodeficiency virus sensory neuropathy. Ann Neurol 69:100-10 (2011). Read more (PubMed: 21280080) »

    See all 5 Publications for this product

    Product Wall

    Please see below for further clarification regarding the following questions:

    Is it OK to extract mitos from frozen tissues?

    A: yes, you can extract mitos from frozen tissues but be aware that freezen/ thaw cycle would weaken the cell...

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    Yes, you can use frozen tissues as sample for all three kits. As a reminder, please follow each individual protocol included with each kit to prepare samples for that particular assay.

    Thank you for contacting us. Yes, we will guarantee ab110169 for use on any mammalian tissues. While it would not qualify for our Abtrial discount program, it will be covered under our Abpromise guarantee. If you have any trouble using this kit on huma...

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    Thank you for contacting us.

    Samples may be prepared as purified mitochondria, crudemitochondria. In most cases whole tissue or cell lysates aresuitable but these may require some sample optimization. Mitochondria samples can be prepared bys...

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    Thank you for your enquiry regarding ab110169: Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer)

    It is not clear at which step the samples got bacterial contaminated.

    Unfortunately, it is difficult to remove bacteria c...

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    Gracias por contactarnos.

    Lamento mucho que el kit no haya llegado en perfecto estado a vuestro laboratorio.

    ¿Podrías por favor confirmarme el número de pedido del kit?

    También agradecerí...

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    Thank you fro your enquiry.

    I am sorry to confirm we would not recommend to use ab109718 Cell Fractionation Kit - HTor ab109719 Cell Fractionation Kit Standardfor isolation of mitochondria from tissue. They are designed for cell culture sing...

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    Please do not use a sonicatorin order to destroy your cells, as this will result in a complete lysis of the mitochondria as well. This means you will not be able to analyse the sample.



    However, we do have a kit in which we deliv...

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    Thank you for your inquiry.

    I am happy to confirm that our lab does not see any specific problems with the experimental set up and buffers your customer is planning to use.

    Cardiac tissue should be an excellent materials for isolati...

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    Thank you for contacting us.

    I have attached the SDS for each kit (Mitochondria Isolation Kit for Tissue) with and without Dounce homogenizer (ab110169 and ab110168), since they are slightly different.

    I hope this information is h...

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    1-10 of 22 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"