Mitochondria分离试剂盒for Cultured Cells (ab110170)
Key features and details
- Assay time: 1 hr
概述
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产品名称
Mitochondria分离试剂盒for Cultured Cells
参阅全部 Mitochondria Isolation 试剂盒 -
检测时间
1h 00m -
种属反应性
与反应: Mammals -
产品概述
ab110170 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Available with a dounce homogenizer as kit ab110171.
Mitochondrial isolation protocol summary:
- suspend cells in reagent A and incubate for 10 min
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant, repeat
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in reagent C and analyze or freeze
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 kit Reagent A 1 x 50ml Reagent B 1 x 50ml Reagent C 1 x 10ml -
研究领域
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别名
- mitochondria purification
- mitochondrial isolation
- mitochondrial purification
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图片
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Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110170 (MS852) Mitochondria Isolation Kit for Cultured Cells were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.
数据表及文件
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SDS download
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Datasheet download
文献 (67)
ab110170 被引用在 67 文献中.
- Suárez-Rivero JM et al. Pterostilbene in Combination With Mitochondrial Cofactors Improve Mitochondrial Function in Cellular Models of Mitochondrial Diseases. Front Pharmacol 13:862085 (2022). PubMed: 35370630
- Morioka E et al. Mitochondrial LETM1 drives ionic and molecular clock rhythms in circadian pacemaker neurons. Cell Rep 39:110787 (2022). PubMed: 35545046
- Toda T et al. Extremely low-frequency pulses of faint magnetic field induce mitophagy to rejuvenate mitochondria. Commun Biol 5:453 (2022). PubMed: 35552531
- Sohn YS et al. A peptide-derived strategy for specifically targeting the mitochondria and ER of cancer cells: a new approach in fighting cancer. Chem Sci 13:6929-6941 (2022). PubMed: 35774163
- Dong L et al. Mannose ameliorates experimental colitis by protecting intestinal barrier integrity. Nat Commun 13:4804 (2022). PubMed: 35974017