概述
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产品名称Anti-Mitochondria抗体[MTC02] (Biotin)
参阅全部 Mitochondria 一抗 -
描述小鼠单克隆抗体[MTC02] to Mitochondria (Biotin)
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宿主Mouse
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偶联物Biotin
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经测试应用适用于: WB, IHC-P, ICC/IFmore details
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种属反应性与反应: Human
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免疫原
Semi-purified mitochondrial preparation.
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阳性对照
- HeLa cells. Liver.
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常规说明
ab79479 produces a spaghetti like staining pattern in the cytoplasm of normal and malignant human cells.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, 10mM PBS, pH 7.4 -
Concentration information loading... -
纯度Protein G purified
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Primary antibody说明ab79479 produces a spaghetti like staining pattern in the cytoplasm of normal and malignant human cells.
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克隆单克隆
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克隆编号MTC02
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同种型IgG1
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab79479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | Use at an assay dependent dilution. |
靶标
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相关性Mitochondria are the power house of the cell. They are distinct organelles with two membranes. Usually they are rod shaped, however they can be round. The outer membrane limits the organelle and the inner membrane is thrown into folds or shelves that project inward and are called "cristae mitochondriales".
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细胞定位Mitochondrial
图片
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondria antibody [MTC02] (Biotin) (ab79479)](http://www.abcam.cn/ps/products/79/ab79479/Images/ab79479-213842-ab79479ihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondria antibody [MTC02] (Biotin) (ab79479)This image is courtesy of an anonymous Abreviewab79479 staining Mitochondria in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with antibody block for 10 minutes; antigen retrieval was by heat mediation in CC1 Mild. Samples were incubated with primary antibody (1/100 in antibody diluent) for 1 hour at 37°C. An undiluted Biotin-conjugated Goat anti-mouse IgG polyclonal was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondria antibody [MTC02] (Biotin) (ab79479)](http://www.abcam.cn/ps/datasheet/images/79/ab79479/Mitochondria-Primary-antibodies-ab79479-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondria antibody [MTC02] (Biotin) (ab79479)IHC image of ab79479 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79479, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
文献
ab79479 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"