MitoBiogenesis™ Western Blot Cocktail (ab123545)

概述

  • 产品名称MitoBiogenesis™ Western Blot Cocktail
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 产品概述

    The MitoBiogenesis™ western blotting cocktail (ab123545) is designed to study the regulation of mitochondrial biogenesis and cellular stress in response to environmental stimuli. It also can be used to monitor drug-induced effects on mitochondrial biogenesis. The two main components of this cocktail target two proteins, which are each subunits of a different oxidative phosphorylation enzyme complex, one subunit I of Complex IV (COX-I), which is mtDNA-encoded, and the 70kDa subunit of Complex II (SDH-A), which is nDNA-encoded. Complex IV includes several proteins which are encoded in the mitochondrion, while the proteins of Complex II are entirely encoded in the nucleus. An anti-Beta actin antibody is used as loading control.

    Cocktail Antibodies:

    Mouse CII-70 (SDHA) [2E3GC12FB2AE2] monoclonal:

    Amount: 25 µg

    Working concentration: 0.5 µg/ml

    Mouse CIV-1 (MT-CO1) [1D6E1A8] monoclonal:

    Amount: 50 µg

    Working concentration: 1 µg/ml

    Mouse Beta actin [mAbcam 8224] monoclonal:

    Amount: 25 µg

    Working concentration: 0.5 µg/ml

  • 说明

    Antibody Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

  • 经测试应用适用于: WBmore details

性能

应用

Our Abpromise guarantee covers the use of ab123545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.

The antibody cocktail should be diluted 250 X to a final working concentration of 2µg/mL for western blotting.

COXI is a highly hydrophobic protein and appears as a broad band at ~35 kDa (not at its true molecular weight at 57 kDa). It is very sensitive to heating. Therefore, the samples, including the positive control, should not be heated over 50°C before loaded on the gel.

MitoBiogenesis™ Western Blot Cocktail 图像

  • All lanes : MitoBiogenesis™ Western Blot Cocktail (ab123545) at 1/250 dilution

    Lane 1 : Human HepG2 cell lysate (vehicle treated)
    Lane 2 : Human HepG2 cell lysate (Chloramphenicol treated)
    Lane 3 : Human HepG2 cell lysate (DDC treated)
    Lane 4 : Human 143B WT cell lysate
    Lane 5 : Human 143B p0 cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    GAM-HRP at 1/3000 dilution
  • All lanes : MitoBiogenesis™ Western Blot Cocktail (ab123545) at 1/250 dilution

    Lane 1 : Human HepG2 cell lysate
    Lane 2 : Human Hela cell lysate
    Lane 3 : Human HDFn cell lysate
    Lane 4 : Human SH-SY5Y cell lysate
    Lane 5 : Rat H9C2 cell lysate
    Lane 6 : Rat H4IIE cell lysate
    Lane 7 : Mouse 3T3 cell lysate
    Lane 8 : Mouse MEF cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    GAM-HRP at 1/3000 dilution

MitoBiogenesis™ Western Blot Cocktail (ab123545)参考文献

This product has been referenced in:
  • Zhou Y  et al. Aristolochic acid causes albuminuria by promoting mitochondrial DNA damage and dysfunction in podocyte. PLoS One 8:e83408 (2013). WB ; Mouse . Read more (PubMed: 24349506) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"