概述

  • 产品名称Anti-MiTF抗体[C5]
    参阅全部 MiTF 一抗
  • 描述
    小鼠单克隆抗体[C5] to MiTF
  • 特异性ab80651 does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay. It does react with both melanocytic and nonmelanocytic isoforms of MiTF.
  • 经测试应用适用于: ICC/IF, IHC-P, Flow Cyt, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Horse, Dog, Human
  • 免疫原

    N-terminal fragment of human MiTF protein.

  • 阳性对照
    • 501 Mel cells, Colo32 cells.

性能

应用

Our Abpromise guarantee covers the use of ab80651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 21646373
IHC-P Use at an assay dependent concentration.
EMSA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 59 kDa.

靶标

  • 功能Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
  • 组织特异性Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells.
  • 疾病相关Defects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
    Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
    Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete.
  • 序列相似性Belongs to the MiT/TFE family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • 翻译后修饰Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BHLHE32 antibody
    • bHLHe32 antibody
    • Class E basic helix-loop-helix protein 32 antibody
    • CMM8 antibody
    • Homolog of mouse microphthalmia antibody
    • Mi antibody
    • Microphthalmia associated transcription factor antibody
    • Microphthalmia, mouse, homolog of antibody
    • Microphthalmia-associated transcription factor antibody
    • MITF antibody
    • MITF_HUMAN antibody
    • mitfa antibody
    • nacre antibody
    • WS2 antibody
    • WS2A antibody
    • z3A.1 antibody
    see all

Anti-MiTF antibody [C5] 图像

  • ab80651 staining MiTF in human melanoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and and DAB chromogen. Note nuclear staining of tumor cells. Staining was performed using antibody containing BSA and azide.
  • Overlay histogram showing Malme-3M cells stained with ab80651 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80651, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Malme-3M cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-MiTF antibody [C5] (ab80651)参考文献

This product has been referenced in:
  • Coelho PA  et al. Over-expression of Plk4 induces centrosome amplification, loss of primary cilia and associated tissue hyperplasia in the mouse. Open Biol 5:N/A (2015). Read more (PubMed: 26701933) »
  • Ohanna M  et al. Senescent cells develop a PARP-1 and nuclear factor-{kappa}B-associated secretome (PNAS). Genes Dev 25:1245-61 (2011). WB, ICC/IF, IHC-P ; Human . Read more (PubMed: 21646373) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Cell (J774A.1 Macrophage)
Specification J774A.1 Macrophage
Permeabilization Yes - 0.5% saponin, 20 min
Fixative Paraformaldehyde
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Verified customer

提交于 Oct 31 2014

Thank you very much for your reply and for sending the additional pictures.

Could you tell me what the positive and negative control tissues were that are shown in the images?

Since the tissue being stained is from rabbit, I would a...

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Thank you for contacting us and for sending all of the protocol information and data. I am sorry that the results with this antibody were notas expected.

I've looked through our records, and we have not received any other recent reports of tr...

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