Anti-MiTF抗体(ab122982)
Key features and details
- Rabbit polyclonal to MiTF
- Suitable for: IHC-Fr, ICC/IF
- Reacts with: Mouse, Chicken
- Isotype: IgG
概述
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产品名称
Anti-MiTF抗体
参阅全部 MiTF 一抗 -
描述
兔多克隆抗体to MiTF -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, ICC/IFmore details -
种属反应性
与反应: Mouse, Chicken -
免疫原
Recombinant full length protein (His-tag) corresponding to Human MiTF.
Database link: O75030 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6
Preservative: 0.05% Sodium azide
Constituent: 99% Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122982于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-Fr |
1/300 - 1/1000.
|
|
ICC/IF |
1/300.
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说明 |
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IHC-Fr
1/300 - 1/1000. |
ICC/IF
1/300. |
靶标
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功能
Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. -
组织特异性
Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. -
疾病相关
Defects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete. -
序列相似性
Belongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻译后修饰
Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 17342 Mouse
- SwissProt: Q08874 Mouse
- Unigene: 333284 Mouse
- Unigene: 454504 Mouse
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别名
- BHLHE32 antibody
- bHLHe32 antibody
- Class E basic helix-loop-helix protein 32 antibody
see all
图片
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Immunohistochemical analysis of RPE (Retinal Pigment Epithelium) in chicken embryo (stage 14) labelling MiTF with ab122982 at dilution of 1/300. Embryo was fixed with paraformaldehyde and embedded in OCT compound and sectioned with a cryostat at 8
μm. Alexa Fluor® 488 conjugated anti-rabbit IgG was used at the secondary antibody.At stage 14, Mitif protein is detected throughout the RPE (Retinal Pigment Epithelium).
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Immunofluorescence analysis of mouse primary melanocytes labelling MiTF with ab122982 at a dilution of 1/300 (right). Nuclei couterstained with DAPI (blue) (left).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab122982 被引用在 16 文献中.
- Sundaramurthi H et al. Uveal Melanoma Cell Line Proliferation Is Inhibited by Ricolinostat, a Histone Deacetylase Inhibitor. Cancers (Basel) 14:N/A (2022). PubMed: 35159049
- Lee J et al. Generation and characterization of hair-bearing skin organoids from human pluripotent stem cells. Nat Protoc 17:1266-1305 (2022). PubMed: 35322210
- Li J et al. The effect of docetaxel on retinal pigment epithelial cells. Toxicol Rep 9:670-678 (2022). PubMed: 35433274
- Yang JM et al. Long-term effects of human induced pluripotent stem cell-derived retinal cell transplantation in Pde6b knockout rats. Exp Mol Med 53:631-642 (2021). PubMed: 33828232
- Zurina IM et al. Human Melanocyte-Derived Spheroids: A Precise Test System for Drug Screening and a Multicellular Unit for Tissue Engineering. Front Bioeng Biotechnol 8:540 (2020). PubMed: 32582665