Anti-MIRO2抗体(ab67588)

概述

  • 产品名称Anti-MIRO2抗体
    参阅全部 MIRO2 一抗
  • 描述
    小鼠多克隆抗体to MIRO2
  • 特异性This antibody has been tested with MIRO2 transfected 293T cell lysate. We have no data on endogenous samples.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein corresponding to Human MIRO2.
    Database link: Q8IXI1

  • 阳性对照
    • MIRO2 transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab67588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 68 kDa.

靶标

  • 功能Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
  • 组织特异性Ubiquitously expressed. Highly expressed in heart, liver, skeletal muscle, kidney and pancreas.
  • 序列相似性Belongs to the mitochondrial Rho GTPase family.
    Contains 2 EF-hand domains.
    Contains 2 Miro domains.
  • 细胞定位Mitochondrion outer membrane. Colocalizes with MGARP and RHOT2 at the mitochondria.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARHT2 antibody
    • C16orf39 antibody
    • hMiro 2 antibody
    • hMiro-2 antibody
    • hMiro2 antibody
    • MIRO-2 antibody
    • MIRO2_HUMAN antibody
    • Mitochondrial Rho GTPase 2 antibody
    • Ras homolog gene family member T2 antibody
    • RASL antibody
    • RHOT2 antibody
    see all

Anti-MIRO2 antibody 图像

  • All lanes : Anti-MIRO2 antibody (ab67588) at 1/500 dilution

    Lane 1 : RHOT2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

Anti-MIRO2 antibody (ab67588)参考文献

ab67588 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for sending the image.

Please keep me updated about the results with the replacement antibody, and let me know if there is anything else that we can do for you.

Have a nice day.

Thank you for your call today and for letting us know about the trouble with ab67588. I apologize again for the previous confusion regarding the expected molecular weight of this protein.

As we discussed, I'm sending a free of charge vial of ...

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Thank you for your inquiry.

I was in touch with the lab and they confirmed that they did not test this product in endogenous samples, only RHOT2 transfected 293T cell lysate.

However, they were able to update us with a new WB imag...

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Thank you for bringing this to our attention. I've been in touch with the lab to see if they have any endogenous testing data.
Here is the subcellular fractionation protocol we talked about on the phone.
http://www.abcam.com/index.html?pageco...

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