antigen sequence, corresponding to amino acids 620-741 of Human MIOS.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Belongs to the WD repeat mio family.
Contains 6 WD repeats.
Information by UniProt
Missing Oocyte, Meiosis Regulator, Homolog (Drosophila) antibody
WD repeat-containing protein mio antibody
Immunocytochemistry/ Immunofluorescence - Anti-MIOS antibody (ab122822)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, cytoplasm and cell junctions. Recommended concentration of ab122822 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIOS antibody (ab122822)
ab122822, at 1/200 dilution, staining MIOS in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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