Anti-Mineralocorticoid Receptor抗体[H3122] (ab41912)


  • 产品名称Anti-Mineralocorticoid Receptor抗体[H3122]
    参阅全部 Mineralocorticoid Receptor 一抗
  • 描述
    小鼠单克隆抗体[H3122] to Mineralocorticoid Receptor
  • 特异性In immunohistochemistry this antibody gives good staining in colon and kidney tissue. However, it has not been possible to detect endogenous expression by Western; as positive control a mouse cell line transfected with cDNA was used for this application.
  • 经测试应用适用于: ELISA, IP, IHC-P, IHC-FoFr, WB, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Bird
    预测可用于: Monkey, Marmoset (common)
  • 免疫原

    Baculovirus-expressed recombinant fragment: ETKGYHSLPE GLDMERRWGQ VSQAVERSSL GPTERTDENN YMEIVNVSCV SGAIPNNSTQ GSSKEKQELL PCLQQDNNRP GILTSDIKTE LESKELSA, corresponding to amino acids 2-99 of Human Mineralocorticoid Receptor.

  • 阳性对照
    • IHC: Human and rat large intestine epithelial cells. WB: COS-7 cell lysate transfected with cDNA



Our Abpromise guarantee covers the use of ab41912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 0.2 µg/ml.
IP Use at an assay dependent concentration.
IHC-P 1/20 - 1/40.
IHC-FoFr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 107 kDa. Block with 5% milk and incubate primary antibody in blocking buffer.
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 1 µg/ml.


  • 功能Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.
  • 组织特异性Ubiquitous. Highly expressed in distal tubules, convoluted tubules and cortical collecting duct in kidney, and in sweat glands. Detected at lower levels in cardiomyocytes, in epidermis and in colon enterocytes.
  • 疾病相关Defects in NR3C2 are a cause of autosomal dominant pseudohypoaldosteronism type I (AD-PHA1) [MIM:177735]. PHA1 is characterized by urinary salt wasting, resulting from target organ unresponsiveness to mineralocorticoids. There are 2 forms of PHA1: the autosomal dominant form that is mild, and the recessive form which is more severe and due to defects in any of the epithelial sodium channel subunits. In AD-PHA1 the target organ defect is confined to kidney. Clinical expression can vary from asymptomatic to moderate. It may be severe at birth, but symptoms remit with age. Familial and sporadic cases have been reported.
    Defects in NR3C2 are a cause of early-onset hypertension with severe exacerbation in pregnancy (EOHSEP) [MIM:605115]. Inheritance is autosomal dominant. The disease is characterized by the onset of severe hypertension before the age of 20, and by suppression of aldosterone secretion.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻译后修饰Phosphorylated.
  • 细胞定位Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Aldosterone receptor antibody
    • MCR antibody
    • MCR_HUMAN antibody
    • MGC133092 antibody
    • Mineralocorticoid receptor antibody
    • MLR antibody
    • MR antibody
    • NR3 C2 antibody
    • NR3C2 antibody
    • NR3C2 protein antibody
    • Nuclear receptor subfamily 3 group C member 2 antibody
    see all

Anti-Mineralocorticoid Receptor antibody [H3122] 图像

  • ab41912 staining Mineralocorticoid in male European Starling brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were transcardially perfused with paraformaldehyde, and immersed in paraformaldyhyde for 24 hours and then 30% sucrose for 24 hours. Slices were cut at a 40 micron thickness. Tissue samples were permeablized with Triton and blocked with 10% serum for 1 hour at 21°C. The sample was incubated with primary antibody (1/500) for 24 hours. A biotin-conjugated Goat anti-mouse IgG monoclonal (1/400) was used as the secondary antibody.

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  • ab41912 staining Mineralocorticoid Receptor in human large intestine paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.
  • ICC/IF image of ab41912 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41912, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab41912 used in Flow Cytometry.
    Human peripheral blood leukocyte cells were fixed with paraformaldehyde, permeabilized with saponin and then stained with ab41912 (which had been directly conjugated to Alexa Fluor 647), used at a 1/50 dilution. Gated on CD3+ lymphocytes (T cells).

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Anti-Mineralocorticoid Receptor antibody [H3122] (ab41912)参考文献

This product has been referenced in:
  • Ji WJ  et al. Spironolactone attenuates bleomycin-induced pulmonary injury partially via modulating mononuclear phagocyte phenotype switching in circulating and alveolar compartments. PLoS One 8:e81090 (2013). ICC/IF ; Mouse . Read more (PubMed: 24260540) »
  • Fraccarollo D  et al. Deletion of cardiomyocyte mineralocorticoid receptor ameliorates adverse remodeling after myocardial infarction. Circulation 123:400-8 (2011). WB ; Mouse . Read more (PubMed: 21242479) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Antigen retrieval step None
Sample Bird Tissue sections (Brain of European Starling)
Specification Brain of European Starling
Permeabilization Yes - Triton
Fixative Paraformaldehyde

Michael Hasstedt

Verified customer

提交于 Oct 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (peripheral blood leukocytes)
Specification peripheral blood leukocytes
Preparation Cell harvesting/tissue preparation method: Whole blood was acquired and erythrocytes were lysed
Sample buffer: BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit
Fixation Paraformaldehyde
Permeabilization Yes - Saponin-based perm buffer from BD (Cytofix/Cytoperm)
Gating Strategy Gated on CD3+ lymphocytes (T cells)

Ms. Luciana Besedovsky

Verified customer

提交于 Jun 14 2011