Anti-Mineralocorticoid Receptor抗体(ab64457)

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概述

  • 产品名称Anti-Mineralocorticoid Receptor抗体
    参阅全部 Mineralocorticoid Receptor 一抗
  • 描述
    兔多克隆抗体to Mineralocorticoid Receptor
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Xenopus laevis, Non human primates
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 950 to the C-terminus of Human Mineralocorticoid Receptor.

    参阅Abcam的专有抗源政策

    (Peptide available as ab74464.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Mouse Kidney Tissue Human Small Intestine Tissue Human Colon Tissue

性能

应用

Our Abpromise guarantee covers the use of ab64457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 107 kDa).
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.
  • 组织特异性Ubiquitous. Highly expressed in distal tubules, convoluted tubules and cortical collecting duct in kidney, and in sweat glands. Detected at lower levels in cardiomyocytes, in epidermis and in colon enterocytes.
  • 疾病相关Defects in NR3C2 are a cause of autosomal dominant pseudohypoaldosteronism type I (AD-PHA1) [MIM:177735]. PHA1 is characterized by urinary salt wasting, resulting from target organ unresponsiveness to mineralocorticoids. There are 2 forms of PHA1: the autosomal dominant form that is mild, and the recessive form which is more severe and due to defects in any of the epithelial sodium channel subunits. In AD-PHA1 the target organ defect is confined to kidney. Clinical expression can vary from asymptomatic to moderate. It may be severe at birth, but symptoms remit with age. Familial and sporadic cases have been reported.
    Defects in NR3C2 are a cause of early-onset hypertension with severe exacerbation in pregnancy (EOHSEP) [MIM:605115]. Inheritance is autosomal dominant. The disease is characterized by the onset of severe hypertension before the age of 20, and by suppression of aldosterone secretion.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻译后修饰Phosphorylated.
  • 细胞定位Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • Aldosterone receptor antibody
    • MCR antibody
    • MCR_HUMAN antibody
    • MGC133092 antibody
    • Mineralocorticoid receptor antibody
    • MLR antibody
    • MR antibody
    • NR3 C2 antibody
    • NR3C2 antibody
    • NR3C2 protein antibody
    • Nuclear receptor subfamily 3 group C member 2 antibody
    see all

Anti-Mineralocorticoid Receptor antibody 图像

  • Western blot - Mineralocorticoid Receptor antibody (ab64457)
    Western blot - Mineralocorticoid Receptor antibody (ab64457)
    All lanes : Anti-Mineralocorticoid Receptor antibody (ab64457) at 1 µg/ml

    Lane 1 : Kidney (Mouse) Tissue Lysate
    Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 3 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 107 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Immunocytochemistry - Mineralocorticoid Receptor antibody (ab64457)
    Immunocytochemistry - Mineralocorticoid Receptor antibody (ab64457)
    ICC/IF image of ab64457 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64457, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2, Hek293 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mineralocorticoid Receptor antibody (ab64457)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mineralocorticoid Receptor antibody (ab64457)
    IHC image of Mineralocorticoid Receptor staining in Human Tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64457, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Anti-Mineralocorticoid Receptor antibody (ab64457)参考文献

This product has been referenced in:
  • Latorre V  et al. Selective ablation of glucocorticoid receptor in mouse keratinocytes increases susceptibility to skin tumorigenesis. J Invest Dermatol 133:2771-9 (2013). Mouse . Read more (PubMed: 23756710) »
  • Lin CH  et al. Reverse Effect of Mammalian Hypocalcemic Cortisol in Fish: Cortisol Stimulates Ca Uptake via Glucocorticoid Receptor-Mediated Vitamin D(3) Metabolism. PLoS One 6:e23689 (2011). WB ; Zebrafish . Read more (PubMed: 21887296) »

See all 2 Publications for this product

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We are observing bands in Western blots using ab64457 on various cell lines that are slightly bigger than overexpressed fullength MR or the bands observed from mouse and rat cortex. I would very much like to know the peptide sequence that was used for ...

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Thank you for your inquiry. Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information. We aim to provide as much information as possible to our customers, so I am sorry that ...

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