Anti-MINA53抗体(ab50908)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to MINA53
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cat, Pig
  • 免疫原

    Synthetic peptide: MPKKAKPTGS GKEEGPAPCK QMKLEAAGGP SALNFDSPSS LFESLISPIK , corresponding to amino acids 1-50 of Human MINA53

  • 阳性对照
    • HepG2 cell lysate; human intestine tissue

性能

应用

Our Abpromise guarantee covers the use of ab50908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 30, 45 kDa (predicted molecular weight: 32, 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

靶标

  • 功能
    Involved in cellular proliferation. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.
  • 组织特异性
    Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.
  • 序列相似性
    Belongs to the MINA53/NO66 family.
    Contains 1 JmjC domain.
  • 细胞定位
    Nucleus. Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 60S ribosomal protein L27a histidine hydroxylase antibody
    • Bifunctional lysine specific demethylase and histidyl hydroxylase MINA antibody
    • FLJ14393 antibody
    • Histone lysine demethylase MINA antibody
    • MDIG antibody
    • MINA antibody
    • MINA_HUMAN antibody
    • Mineral dust-induced gene protein antibody
    • Myc induced nuclear antigen 53 kDa antibody
    • MYC induced nuclear antigen antibody
    • MYC-induced nuclear antigen antibody
    • NO52 antibody
    • Nucleolar protein 52 antibody
    • Ribosomal oxygenase MINA antibody
    • ROX antibody
    see all

Anti-MINA53 antibody 图像

  • Anti-MINA53 antibody (ab50908) at 1.25 µg/ml + HepG2 cell Lysate (10µg)

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32, 53 kDa
    Observed band size : 30,45 kDa (why is the actual band size different from the predicted?)
  • ab50908 at a concentration of 4-8µg/ml positively labelling epithelial cells of human intestinal villus(indicated by arrows) in paraffin-embedded tissue. Magnification 400X
  • Immunohistochemistry of Human kidney lysate tissue at an antibody concentration of 4-8µg/ml using anti-MINA antibody (ab50908)

Anti-MINA53 antibody (ab50908)参考文献

ab50908 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH8, 100C, 20 minutes
Sample
Human Tissue sections (colon cancer)
Specification
colon cancer
Permeabilization
No
Fixative
Formaldehyde
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提交于 May 13 2014

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