Anti-MINA53抗体(ab37175)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to MINA53
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human MINA53.

    (Peptide available as ab37300.)

  • 阳性对照
    • ab37175 gave a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line)

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab37175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

靶标

  • 功能
    Involved in cellular proliferation. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.
  • 组织特异性
    Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.
  • 序列相似性
    Belongs to the MINA53/NO66 family.
    Contains 1 JmjC domain.
  • 细胞定位
    Nucleus. Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 60S ribosomal protein L27a histidine hydroxylase antibody
    • Bifunctional lysine specific demethylase and histidyl hydroxylase MINA antibody
    • FLJ14393 antibody
    • Histone lysine demethylase MINA antibody
    • MDIG antibody
    • MINA antibody
    • MINA_HUMAN antibody
    • Mineral dust-induced gene protein antibody
    • Myc induced nuclear antigen 53 kDa antibody
    • MYC induced nuclear antigen antibody
    • MYC-induced nuclear antigen antibody
    • NO52 antibody
    • Nucleolar protein 52 antibody
    • Ribosomal oxygenase MINA antibody
    • ROX antibody
    see all

图片

  • All lanes : Anti-MINA53 antibody (ab37175) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab37175 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa)
Total protein in input
400 µg
Specification
HeLa
Immuno-precipitation step
Protein A
Username

Mr. Antibody Solutions

Verified customer

提交于 Apr 10 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293, HeLa, Jurkat, U2OS)
Loading amount
20 µg
Specification
HEK 293, HeLa, Jurkat, U2OS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Apr 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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