Anti-MINA53抗体(ab37175)

概述

  • 产品名称Anti-MINA53抗体
    参阅全部 MINA53 一抗
  • 描述
    兔多克隆抗体to MINA53
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human MINA53.

    (Peptide available as ab37300.)

  • 阳性对照
    • ab37175 gave a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab37175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

靶标

  • 功能Involved in cellular proliferation. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.
  • 组织特异性Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.
  • 序列相似性Belongs to the MINA53/NO66 family.
    Contains 1 JmjC domain.
  • 细胞定位Nucleus. Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 60S ribosomal protein L27a histidine hydroxylase antibody
    • Bifunctional lysine specific demethylase and histidyl hydroxylase MINA antibody
    • FLJ14393 antibody
    • Histone lysine demethylase MINA antibody
    • MDIG antibody
    • MINA antibody
    • MINA_HUMAN antibody
    • Mineral dust-induced gene protein antibody
    • Myc induced nuclear antigen 53 kDa antibody
    • MYC induced nuclear antigen antibody
    • MYC-induced nuclear antigen antibody
    • NO52 antibody
    • Nucleolar protein 52 antibody
    • Ribosomal oxygenase MINA antibody
    • ROX antibody
    see all

Anti-MINA53 antibody 图像

  • All lanes : Anti-MINA53 antibody (ab37175) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.

Anti-MINA53 antibody (ab37175)参考文献

ab37175 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa)
Total protein in input 400 µg
Specification HeLa
Immuno-precipitation step Protein A
Username

Mr. Antibody Solutions

Verified customer

提交于 Apr 10 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK 293, HeLa, Jurkat, U2OS)
Loading amount 20 µg
Specification HEK 293, HeLa, Jurkat, U2OS
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Apr 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"