概述

  • 产品名称Anti-MIG抗体
    参阅全部 MIG 一抗
  • 描述
    兔多克隆抗体to MIG
  • 经测试应用适用于: ELISA, Neutralising, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Highly pure (>98%) recombinant hMIG (human Monokine induced by Interferon-Gamma)

性能

  • 形式Lyophilised:Reconstitute with 200µl of sterile water.
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型unknown
  • 轻链类型unknown
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab9720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 0.5 µg/ml.
Neutralising Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hMIG (100.00 ng/ml), a concentration of 5.0 - 7.0 µg/ml of this antibody is required
WB Use 0.1µg for 100.2 cells.
IHC-P Use a concentration of 0.25 - 0.5 µg/ml.

靶标

  • 功能Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C-X-C motif chemokine 9 antibody
    • chemokine (C-X-C motif) ligand 9 antibody
    • CMK antibody
    • crg-10 antibody
    • CXCL9 antibody
    • CXCL9_HUMAN antibody
    • gamma interferon induced monokine antibody
    • Gamma-interferon-induced monokine antibody
    • HuMIG antibody
    • MIG antibody
    • monokine induced by gamma interferon antibody
    • monokine induced by interferon gamma antibody
    • Monokine induced by interferon-gamma antibody
    • SCYB9 antibody
    • Small inducible cytokine B9 antibody
    • small inducible cytokine subfamily B member 9 antibody
    • Small-inducible cytokine B9 antibody
    see all

Anti-MIG antibody 图像

  • ab9720 staining MIG in human renal tumor with parenchyma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

Anti-MIG antibody (ab9720)参考文献

This product has been referenced in:
  • Chmielewski S  et al. STAT1-dependent signal integration between IFN? and TLR4 in vascular cells reflect pro-atherogenic responses in human atherosclerosis. PLoS One 9:e113318 (2014). IHC-P ; Human . Read more (PubMed: 25478796) »
  • Xu J  et al. Detection of severe acute respiratory syndrome coronavirus in the brain: potential role of the chemokine mig in pathogenesis. Clin Infect Dis 41:1089-96 (2005). Read more (PubMed: 16163626) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. The product is shipped as 100 ug of lyophilized antibody. If reconstituted in 200 ul of water, the concentration will be 0.5 mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if...

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Thank you for contacting us. We do not currently have any feedback from customers or lab testing regarding whether it would be possible to conjugate either of these antibodies to gold, and we therefore do not have any recommendations for a pH or conce...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Tonsil (periphery))
Specification Tonsil (periphery)
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step (agent) for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. R Berahovich

Verified customer

提交于 Dec 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colorectal carcinoma)
Specification Colorectal carcinoma
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jun 10 2011

Thank you for contacting us for technical support regarding ab9720. This antibody has not been tested on mouse tissue therefore we do not know what sort of banding pattern it gives with MIG of this species I'm afraid. There is 73% homology between the ...

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Unfortunately, this antibody has not been tested for IHC in frozen sections, so we can't guarantee that it will work.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"