概述

  • 产品名称Anti-MIF4GD抗体
    参阅全部 MIF4GD 一抗
  • 描述
    兔多克隆抗体to MIF4GD
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide: FVLIRDGFLL PTGLSSLAQL LLLEIIEFRA AGWKTTPAAH KYYYSEVSD, corresponding to C terminal amino acids 208-256 of Human MIF4GD

  • 阳性对照
    • HepG2 cell lysate, Human kidney

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab51242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA 1/312500.

Titre using peptide based assay 1/312500.

靶标

Anti-MIF4GD antibody 图像

  • Anti-MIF4GD antibody (ab51242) at 1.25 µg/ml + HepG2 cell lysate at 10 µg with skim milk/ PBS at 5 %

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)
  • ab51242 at 4.0 µg/ml staining epithelial cells of renal tubule from human kidney; paraffin embedded, magnification 400x.

Anti-MIF4GD antibody (ab51242)参考文献

ab51242 has not yet been referenced specifically in any publications.

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