概述

  • 产品名称
  • 描述
    兔多克隆抗体to MIF
  • 经测试应用
    适用于: ICC/IF, ELISA, IHC-Fr, WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Recombinant full length protein (Rat)(E. coli-expressed).

  • 阳性对照
    • LPS stimulated RAW cell lysate Neuro2A (mouse neuroblastoma cell line) lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/250.
ELISA Use at an assay dependent concentration. PubMed: 19066630
IHC-Fr Use at an assay dependent concentration.
WB 1/2000.
IP 1/500.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
  • 疾病相关
    Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
  • 序列相似性
    Belongs to the MIF family.
  • 细胞定位
    Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GIF antibody
    • GLIF antibody
    • Glycosylation inhibiting factor antibody
    • Glycosylation-inhibiting factor antibody
    • L-dopachrome isomerase antibody
    • L-dopachrome tautomerase antibody
    • Macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody
    • Macrophage migration inhibitory factor antibody
    • MIF antibody
    • MIF protein antibody
    • MIF_HUMAN antibody
    • MMIF antibody
    • Phenylpyruvate tautomerase antibody
    see all

Anti-MIF antibody 图像

  • Anti-MIF antibody (ab7207) + LPS stimulated RAW cell lysate

    Performed under reducing conditions.

    MIF detected by Western Blot in LPS stimulated RAW cell lysate.

  • ab7207 staining MIF in Mouse small intestine tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with Dako protein block for 1 hour at room temperature. Samples were incubated with primary antibody (1/200) for 1 hour. An undiluted Alkaline Phosphatase-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • ab7207 staining MIF in Rat liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% protein blocking solution for 1 hour at room temperature; antigen retrieval was by heat mediation with a citrate EDTA buffer (pH 6.2). Samples were incubated with primary antibody (1/200 in antibody diluent) for 1 hour. An undiluted Alkaline phosphatase-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • ab7207 staining MIF in murine immortalised bone marrow-derived macrophages by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/250 in 5% goat serum/3% BSA in PBS) for 1 hour at 20°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.
  • All lanes : Anti-MIF antibody (ab7207) at 1/1000 dilution

    Lane 1 : Whole cell lysate prepared from normal rat serum
    Lane 2 : Whole cell lysate prepared from normal rat serum
    Lane 3 : Whole cell lysate prepared from rat serum (90 minutes warm ischemia and 1h reperfusion)
    Lane 4 : Whole cell lysate prepared from rat serum (90 minutes warm ischemia and 1h reperfusion)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
    Developed using the ECL technique

    Observed band size : 13 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

  • From a review by Arsi Rosengren.
  • ab7207 staining mouse Testis tissue sections by Immunohistochemistry (Frozen sections). Tissue was fixed in paraformaldehyde and blocked in 1% serum for 10 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with sample for 20 hours at 20°C. A biotin labelled goat polyclonal to rabbit IgG antibody, diluted 1/500 was used as the secondary.

    See Abreview

  • ab7207 used at a 1/1000 dilution as detection antibody in ELISA testing MIF in rat serum samples. Samples were blocked with 1% BSA for 2 hours at room temperature. The secondary used was an HRP conjugated donkey anti-goat (H+L) polyclonal used at a 1/200,000 dilution.

    See Abreview

Anti-MIF antibody (ab7207)参考文献

This product has been referenced in:
  • Zhang YY  et al. Proteomics reveals potential non-neuronal cholinergic receptor-effectors in endothelial cells. Acta Pharmacol Sin 35:1137-49 (2014). Read more (PubMed: 25088000) »
  • Zaghloul N  et al. Overexpression of Extracellular Superoxide Dismutase Protects against Brain Injury Induced by Chronic Hypoxia. PLoS One 9:e108168 (2014). WB ; Mouse . Read more (PubMed: 25268361) »

See all 14 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (immortalised bone marrow-derived macrophages)
Specification
immortalised bone marrow-derived macrophages
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. James Harris

Verified customer

提交于 Aug 24 2012

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Rat Serum (serum)
Specification
serum
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Type
Sandwich (Capture)
Username

Abcam user community

Verified customer

提交于 Feb 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (liver)
Specification
liver
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Feb 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (small intestine)
Specification
small intestine
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Feb 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Serum)
Loading amount
10 µg
Specification
Serum
Treatment
90 minutes warm ischemia and 1h reperfusion
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jun 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate EDTA buffer pH=6.2
Permeabilization
No
Blocking step
Dako Protein block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100%
Username

Abcam user community

Verified customer

提交于 May 31 2011

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Loading amount
30 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Apr 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate EDTA pH6,2
Permeabilization
No
Blocking step
Protein Block solution Dako as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Mar 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Loading amount
30 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jan 10 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (liver)
Loading amount
30 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jan 07 2011

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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