概述

  • 产品名称Anti-MIF抗体
    参阅全部 MIF 一抗
  • 描述
    兔多克隆抗体to MIF
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human MIF (C terminal). A synthetic peptide corresponding to a sequence at the C-terminus of human MIF, different from the related rat and mouse sequences by one amino acid.

  • 阳性对照
    • Rat kidney tissue, mammary cancer tissue lysate.

性能

  • 形式Lyophilised:0.2ml of distilled water will yield a concentration of 500µg/ml.
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide, 0.01% Thimerosal (merthiolate)
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab65869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
  • 疾病相关Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
  • 序列相似性Belongs to the MIF family.
  • 细胞定位Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
  • Information by UniProt
  • 数据库链接
  • 别名
    • acrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody
    • GIF antibody
    • GLIF antibody
    • Glycosylation inhibiting factor antibody
    • Glycosylation-inhibiting factor antibody
    • L-dopachrome isomerase antibody
    • L-dopachrome tautomerase antibody
    • Macrophage migration inhibitory factor antibody
    • MIF antibody
    • MIF protein antibody
    • MIF_HUMAN antibody
    • MMIF antibody
    • Phenylpyruvate tautomerase antibody
    see all

Anti-MIF antibody 图像

  • This image shows rat kidney tissue stained with ab65869 at 2µg/ml. Cytoplasmic staining of MIF, using DAB.
  • All lanes : Anti-MIF antibody (ab65869)

    Lane 1 : HeLa cell lysate
    Lane 2 : COLO320 cell lysate
    Lane 3 : MM231 cell lysate


    Predicted band size : 13 kDa
  • Anti-MIF antibody (ab65869) at 2 µg/ml + mammary cancer tissue lysate

    Predicted band size : 13 kDa
    Observed band size : 13 kDa

    Secondary antibody - anti-rabbit HRP (ab6721)

Anti-MIF antibody (ab65869)参考文献

ab65869 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Liver)
Loading amount 30 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jun 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate EDTA buffer pH=6.2
Permeabilization No
Blocking step Protein Block Dako as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Mar 30 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (liver)
Loading amount 30 µg
Specification liver
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 12 2010

Application Immunocytochemistry
Sample Rat Cell (peritoneal macrophages)
Specification peritoneal macrophages
Fixative Methanol
Permeabilization Yes - 0.1% Triton x-100
Blocking step No blocking step used for 1 hour(s) and 0 minute(s) · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jan 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"