概述

  • 产品名称
  • 描述
    兔多克隆抗体to MIF
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human MIF (C terminal). A synthetic peptide corresponding to a sequence at the C-terminus of human MIF, different from the related rat and mouse sequences by one amino acid.

  • 阳性对照
    • Rat kidney tissue, mammary cancer tissue lysate.

性能

  • 形式
    Lyophilised:0.2ml of distilled water will yield a concentration of 500µg/ml.
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide, 0.01% Thimerosal (merthiolate)
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab65869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能
    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
  • 疾病相关
    Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
  • 序列相似性
    Belongs to the MIF family.
  • 细胞定位
    Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GIF antibody
    • GLIF antibody
    • Glycosylation inhibiting factor antibody
    • Glycosylation-inhibiting factor antibody
    • L-dopachrome isomerase antibody
    • L-dopachrome tautomerase antibody
    • Macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody
    • Macrophage migration inhibitory factor antibody
    • MIF antibody
    • MIF protein antibody
    • MIF_HUMAN antibody
    • MMIF antibody
    • Phenylpyruvate tautomerase antibody
    see all

图片

  • This image shows rat kidney tissue stained with ab65869 at 2µg/ml. Cytoplasmic staining of MIF, using DAB.
  • All lanes : Anti-MIF antibody (ab65869)

    Lane 1 : HeLa cell lysate
    Lane 2 : COLO320 cell lysate
    Lane 3 : MM231 cell lysate


    Predicted band size : 13 kDa
  • Anti-MIF antibody (ab65869) at 2 µg/ml + mammary cancer tissue lysate

    Predicted band size : 13 kDa
    Observed band size : 13 kDa

    Secondary antibody - anti-rabbit HRP (ab6721)

文献

This product has been referenced in:
  • Guo F  et al. Identification of differentially expressed inflammatory factors in Wilms tumors and their association with patient outcomes. Oncol Lett 14:687-694 (2017). Read more (PubMed: 28693222) »

See 1 Publication for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Liver)
Loading amount
30 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jun 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate EDTA buffer pH=6.2
Permeabilization
No
Blocking step
Protein Block Dako as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Mar 30 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (liver)
Loading amount
30 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 12 2010

Application
Immunocytochemistry
Sample
Rat Cell (peritoneal macrophages)
Specification
peritoneal macrophages
Fixative
Methanol
Permeabilization
Yes - 0.1% Triton x-100
Blocking step
No blocking step used for 1 hour(s) and 0 minute(s) · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jan 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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