概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to MIF
  • 经测试应用
    适用于: IHC-P, WB, Flow Cytmore details
  • 种属反应性
    与反应: Human, Recombinant fragment
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-116 of Human MIF

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab55445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
  • 疾病相关
    Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
  • 序列相似性
    Belongs to the MIF family.
  • 细胞定位
    Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GIF antibody
    • GLIF antibody
    • Glycosylation inhibiting factor antibody
    • Glycosylation-inhibiting factor antibody
    • L-dopachrome isomerase antibody
    • L-dopachrome tautomerase antibody
    • Macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody
    • Macrophage migration inhibitory factor antibody
    • MIF antibody
    • MIF protein antibody
    • MIF_HUMAN antibody
    • MMIF antibody
    • Phenylpyruvate tautomerase antibody
    see all

Anti-MIF antibody 图像

  • MIF antibody (ab55445) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human lung, adenosqumous cell carcinoma tissue.
  • Western blot against tagged recombinant protein immunogen using ab55445 MIF antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa
  • Overlay histogram showing Jurkat cells stained with ab55445 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55445, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunohistochemical analysis of Human nasopharyngeal carcinoma, staining MIF with ab55445 at 1/100 dilution.

Anti-MIF antibody (ab55445)参考文献

This product has been referenced in:
  • Kassaar O  et al. Macrophage Migration Inhibitory Factor is subjected to glucose modification and oxidation in Alzheimer's Disease. Sci Rep 7:42874 (2017). Read more (PubMed: 28230058) »
  • Zeng J  et al. Transient transfection of macrophage migration inhibitory factor small interfering RNA disrupts the biological behavior of oral squamous carcinoma cells. Mol Med Rep 13:174-80 (2016). Read more (PubMed: 26549761) »

See all 11 Publications for this product

Product Wall

Thank you very much for contacting us with your enquiry.

In Western blot, this antibody has been tested against the recombinant Mif protein, so I would recommend using ab73230 for a positive control in WB-

http://www.abcam.com/MIF-p...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - 0.025% Triton X-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Username

Ms. Kristine Jun

Verified customer

提交于 Mar 30 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Human Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.1% Tween-20 in PBS washes
Fixative
Paraformaldehyde
Username

Ms. Kristine Jun

Verified customer

提交于 Feb 22 2012

DISCOUNT CODE: Expiration date: I am very pleased to hear you would like to accept our offer and test ab55445 in IHC-FR on human tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit a...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册