概述

  • 产品名称Anti-MIF抗体
    参阅全部 MIF 一抗
  • 描述
    鸡多克隆抗体to MIF
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Cow, Dog, Pig, Cynomolgus Monkey, Macaque Monkey
  • 免疫原

    Synthetic peptide: PMFIVNTNV PRASVPDGFL SELTQQLAQA TG conjugated to KLH, corresponding to amino acids 2-32 of Human MIF

  • 阳性对照
    • Supernatants or lysates of endotoxin-stimulated human peripheral blood mononuclear cells (PBMC).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • 纯度IgY fraction
  • 纯化说明This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.
  • 克隆多克隆
  • 同种型IgY
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab34644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000 - 1/2000.
WB 1/500 - 1/1000. Detects a band of approximately 12.3 kDa (predicted molecular weight: 14 kDa).

靶标

  • 功能Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
  • 疾病相关Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
  • 序列相似性Belongs to the MIF family.
  • 细胞定位Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
  • Information by UniProt
  • 数据库链接
  • 别名
    • acrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody
    • GIF antibody
    • GLIF antibody
    • Glycosylation inhibiting factor antibody
    • Glycosylation-inhibiting factor antibody
    • L-dopachrome isomerase antibody
    • L-dopachrome tautomerase antibody
    • Macrophage migration inhibitory factor antibody
    • MIF antibody
    • MIF protein antibody
    • MIF_HUMAN antibody
    • MMIF antibody
    • Phenylpyruvate tautomerase antibody
    see all

Anti-MIF antibody 图像

  • All lanes : Anti-MIF antibody (ab34644) at 1/500 dilution

    Lane 1 : Control
    Lane 2 : 100 ng of recombinant MIF present in a bacterial lysate

    Secondary
    Lane 2 : Goat anti chicken at 1/5000 dilution


    Predicted band size : 14 kDa
    Observed band size : 12.3 kDa (why is the actual band size different from the predicted?)

Anti-MIF antibody (ab34644)参考文献

ab34644 has not yet been referenced specifically in any publications.

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