Anti-MIF抗体[2Ar3] (ab14575)

概述

  • 产品名称Anti-MIF抗体[2Ar3]
    参阅全部 MIF 一抗
  • 描述
    小鼠单克隆抗体[2Ar3] to MIF
  • 特异性Recognizes recombinant and natural human Migration Inhibitory Factor (MIF). Based on direct ELISA, shows no crossreactivity with most cytokines.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    E. coli-derived recombinant full length protein (Human).

  • 常规说明= 10ng/mg Endotoxin. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: BSA, PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号2Ar3
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab14575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: Use at a concentration of 4 ug/ml for direct ELISA and 2 ug/ml for capture in sandwich ELISA.

    WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 13.8 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
    • 疾病相关Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    • 序列相似性Belongs to the MIF family.
    • 细胞定位Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
    • Information by UniProt
    • 数据库链接
    • 别名
      • acrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody
      • GIF antibody
      • GLIF antibody
      • Glycosylation inhibiting factor antibody
      • Glycosylation-inhibiting factor antibody
      • L-dopachrome isomerase antibody
      • L-dopachrome tautomerase antibody
      • Macrophage migration inhibitory factor antibody
      • MIF antibody
      • MIF protein antibody
      • MIF_HUMAN antibody
      • MMIF antibody
      • Phenylpyruvate tautomerase antibody
      see all

    Anti-MIF antibody [2Ar3] (ab14575)参考文献

    ab14575 has not yet been referenced specifically in any publications.

    Product Wall

    The monoclonal antibody ab14575 was raised using human MIF, recombinant protein expressed in E. coli. The epitope has not been determined. For a sandwich ELISA, I recommend ab14575 for capture and goat polyclonal ab14574 for detection, followed by ...

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    Thank you for your enquiry. I have found reference to the use of LPS stimulated RAW cell lysate, Neuro2A (mouse neuroblastoma cell line) lysate or a Human Thymus lysate for use as a positive control for antibodies against MIF. Unfortunately we do...

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    Thank you for your email. If you are interested in ordering multiple vials of the same antibody, I can offer you a discount. However, if you are interested in ordering one each of the 5 antibodies that you listed, I cannot offer you a discount. Shippin...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"