The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 50 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
MIER1 (previously called er1) was first isolated from Xenopus laevis embryonic cells as a novel fibroblast growth factor. Human MIER1 (hMIER1) was shown to encode a nuclear protein with an N terminal acidic transcription activation domain. The human orthologue of MIER1 displays 91% similarity to the Xenopus sequence at the amino acid level and was shown to be upregulated in breast carcinoma cell lines and tumors. Alternative splicing at the 3' end of hMIER1 produces two major isoforms, hMIER1 and hMIER1ß, which contain distinct C terminal domains.
The association of hMIER1 with Sp1 represents a novel mechanism for the negative regulation of Sp1 target promoters. Results demonstrate that alternate use of a facultative intron regulates the subcellular localization of hMIER1 proteins and this may have important implications for hMIER1 function in the regulation of transcription. Repressor activity is due to interaction and recruitment of a trichostatin A sensitive histone deacetylase 1 (HDAC1).