概述

  • 产品名称Anti-MIER1抗体
  • 描述
    兔多克隆抗体to MIER1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide: KDASRRTGDE KGVEAIPEGS HIKDNEQALY ELVKCNFDTE EALRRLRFNV, corresponding to internal sequence amino acids 253-302 of Human MIER1

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab26254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 50 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

靶标

  • 相关性MIER1 (previously called er1) was first isolated from Xenopus laevis embryonic cells as a novel fibroblast growth factor. Human MIER1 (hMIER1) was shown to encode a nuclear protein with an N terminal acidic transcription activation domain. The human orthologue of MIER1 displays 91% similarity to the Xenopus sequence at the amino acid level and was shown to be upregulated in breast carcinoma cell lines and tumors. Alternative splicing at the 3' end of hMIER1 produces two major isoforms, hMIER1 and hMIER1ß, which contain distinct C terminal domains. The association of hMIER1 with Sp1 represents a novel mechanism for the negative regulation of Sp1 target promoters. Results demonstrate that alternate use of a facultative intron regulates the subcellular localization of hMIER1 proteins and this may have important implications for hMIER1 function in the regulation of transcription. Repressor activity is due to interaction and recruitment of a trichostatin A sensitive histone deacetylase 1 (HDAC1).
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • ER1 antibody
    • Mesoderm induction early response 1 antibody
    • Mesoderm induction early response 1 homolog (Xenopus laevis) antibody
    • MI-ER1 antibody

Anti-MIER1 antibody 图像

Anti-MIER1 antibody (ab26254)参考文献

ab26254 has not yet been referenced specifically in any publications.

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