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RabMAb

Anti-Midkine抗体[EP1143Y] (ab52637)

概述

  • 产品名称
    Anti-Midkine抗体[EP1143Y]
    参阅全部 Midkine 一抗
  • 描述
    兔单克隆抗体[EP1143Y] to Midkine
  • 经测试应用
    适用于: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Midkine aa 100 to the C-terminus (C terminal).

  • 阳性对照
    • Human liver carcinoma; Human pancreatic carcinoma; HeLa cells; SH-SY5Y (Human neuroblastoma cell line from bone marrow).
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab52637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).

For unpurified use at 1/500.

IP 1/20.

For unpurified use at 1/30. 

Flow Cyt Use at an assay dependent concentration.
IHC-P 1/50.
ICC/IF 1/100.

For unpurified use at 1/250 - 1/500.

靶标

  • 功能
    Has heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.
  • 序列相似性
    Belongs to the pleiotrophin family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amphiregulin associated protein antibody
    • Amphiregulin-associated protein antibody
    • ARAP antibody
    • FLJ27379 antibody
    • Mdk antibody
    • Midgestation and kidney protein antibody
    • Midkine antibody
    • MK 1 antibody
    • MK antibody
    • MK_HUMAN antibody
    • MK1 antibody
    • NEGF 2 antibody
    • NEGF2 antibody
    • Neurite growth promoting factor 2 antibody
    • Neurite outgrowth promoting protein antibody
    • Neurite outgrowth-promoting factor 2 antibody
    • Neurite outgrowth-promoting protein antibody
    • Retinoic acid inducible factor antibody
    see all

Anti-Midkine antibody [EP1143Y] 图像

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Midkine with purified ab52637 at 1:100 dilution (10.5μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Anti-Midkine antibody [EP1143Y] (ab52637) at 1/500 dilution (unpurified) + Recombinant protein at 0.001 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size : 16 kDa
    Observed band size : 16 kDa
  • ab52637 (purified) at 1:20 dilution (2ug) immunoprecipitating Midkine in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate.

    Lane 1 (input): SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate 10ug

    Lane 2 (+): ab52637 & SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52637 in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:5000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

  • Human liver carcinoma stained with unpurified ab52637 at 1/100 - 1/250 dilution



  • Predicted band size : 16 kDa

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreatic carcinoma tissue sections labeling Midkine with purified ab52637 at 1:50 dilution (2.1 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

  • Anti-Midkine antibody [EP1143Y] (ab52637) at 1/1000 dilution (purified) + SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 16 kDa
    Observed band size : 16 kDa

    Blocking and diluting: 5% NFDM/TBST

  • Anti-Midkine antibody [EP1143Y] (ab52637) at 1/10000 dilution (purified) + Recombinant Human Midkine protein (ab49848) at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 16 kDa
    Observed band size : 16 kDa

    Blocking and diluting buffer: 5% NFDM/TBST

  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Midkine with purified ab52637 at 1/250 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • HeLa cells stained with unpurified ab52637 at 1/250 - 1/500 dilution

Anti-Midkine antibody [EP1143Y] (ab52637)参考文献

This product has been referenced in:
  • Yamashita T  et al. Serum midkine as a biomarker for malignancy, prognosis, and chemosensitivity in head and neck squamous cell carcinoma. Cancer Med 5:415-25 (2016). IHC-P ; Human . Read more (PubMed: 26798989) »
  • Yuan K  et al. MDK Protein Overexpression Correlates with the Malignant Status and Prognosis of Non-small Cell Lung Cancer. Arch Med Res 46:635-41 (2015). WB, IHC-P ; Human . Read more (PubMed: 26656665) »

See all 8 Publications for this product

Product Wall

Application
Western blot
Sample
Chicken Tissue lysate - whole (E1.5 embryos)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
10 µg
Specification
E1.5 embryos
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

提交于 Jan 23 2017

Thank you for contacting us. This product is a tissue culture supernatant, not a purified antibody. Concentrations are generally not determined for tissue culture supernatant products as they are not relevance; they may contain other proteins aside fro...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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