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Our Abpromise guarantee covers the use of ab110614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Midkine.|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Midkine (Biotin) (ab83160). Using ab83160 as the detection antibody and ab110614 as the capture antibody, the detection limit for recombinant Human Midkine is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 16 kDa. The detection limit for recombinant Human Midkine is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.|