, corresponding to amino acids 703-715 of Human MID2.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml.
Use at an assay dependent concentration.
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 81 kDa.Can be blocked with
Human MID2 peptide (ab23250).
Approx 80-85kDa band observed in Mouse and Rat Heart, and Rat Kidney lysates (calculated MW of 77.8kDa according to Mouse NP_035975.1 and 83.1kDa according to Rat NP_001178818.1).
Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine.
Protein modification; protein ubiquitination.
Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers. Contains 1 B30.2/SPRY domain. Contains 1 COS domain. Contains 1 fibronectin type-III domain. Contains 1 RING-type zinc finger.
The tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) mediates dimerization.
Associates with microtubules in a manner that is dependent on the C-terminal B30.2 domain.
Phosphorylated on serine and threonine residues.
Cytoplasm. Cytoplasm > cytoskeleton. Microtubule-associated.
Information by UniProt
has not yet been referenced specifically in any publications.
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