概述

  • 产品名称Anti-MID2抗体
    参阅全部 MID2 一抗
  • 描述
    山羊多克隆抗体to MID2
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Cow, Pig
  • 免疫原

    Synthetic peptide:

    QESPYVSGMKTCH

    , corresponding to C terminal amino acids 723-735 of Human MID2 (NP_036348.2).

  • 阳性对照
    • Human kidney tissue; Mouse heart lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab115604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 83 kDa.
ELISA 1/32000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 组织特异性Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 B30.2/SPRY domain.
    Contains 1 COS domain.
    Contains 1 fibronectin type-III domain.
    Contains 1 RING-type zinc finger.
  • 结构域The tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) mediates dimerization.
    Associates with microtubules in a manner that is dependent on the C-terminal B30.2 domain.
  • 翻译后修饰Phosphorylated on serine and threonine residues.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton. Microtubule-associated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ37715 antibody
    • FLJ41813 antibody
    • FXY2 antibody
    • MID2 antibody
    • Midin 2 antibody
    • Midin-2 antibody
    • Midline 2 antibody
    • Midline defect 2 antibody
    • Midline-2 antibody
    • probable E3 ubiquitin protein ligase MID2 antibody
    • Probable E3 ubiquitin-protein ligase MID2 antibody
    • RING finger protein 60 antibody
    • Ring finger protein protein 60 antibody
    • RNF60 antibody
    • TRIM1 antibody
    • TRIM1_HUMAN antibody
    • tripartite motif containing protein 1 antibody
    • Tripartite motif protein 1 antibody
    • Tripartite motif-containing protein 1 antibody
    see all

Anti-MID2 antibody 图像

  • ab115604, at 5µg/ml, staining MID2 in Formalin-fixed, Paraffin-embedded Human Kidney tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-MID2 antibody (ab115604) at 1 µg/ml + Mouse heart lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 83 kDa

Anti-MID2 antibody (ab115604)参考文献

ab115604 has not yet been referenced specifically in any publications.

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