The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 83 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
组织特异性Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine.
通路Protein modification; protein ubiquitination.
序列相似性Belongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 B30.2/SPRY domain. Contains 1 COS domain. Contains 1 fibronectin type-III domain. Contains 1 RING-type zinc finger.
结构域The tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) mediates dimerization. Associates with microtubules in a manner that is dependent on the C-terminal B30.2 domain.
翻译后修饰Phosphorylated on serine and threonine residues.
ab115604, at 5µg/ml, staining MID2 in Formalin-fixed, Paraffin-embedded Human Kidney tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-MID2 antibody (ab115604)
Anti-MID2 antibody (ab115604) at 1 µg/ml + Mouse heart lysate at 35 µg developed using the ECL technique
Predicted band size : 83 kDa
Anti-MID2 antibody (ab115604)参考文献
has not yet been referenced specifically in any publications.