概述

  • 产品名称
    Anti-MID1抗体
  • 描述
    兔多克隆抗体to MID1
  • 经测试应用
    适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of human MID1

  • 阳性对照
    • 293 cell extracts, human brain tissue and HeLa cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab70770 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab70770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能
    May have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation.
  • 组织特异性
    In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain.
  • 疾病相关
    Defects in MID1 are the cause of Opitz syndrome type I (OS-I) [MIM:300000]. OS-I is an X-linked recessive disorder characterized by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, lip-palate-laryngotracheal clefts, imperforate anus, developmental delay and congenital heart defects. OS-I mutations produce proteins with a decreased affinity for microtubules.
  • 序列相似性
    Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 B30.2/SPRY domain.
    Contains 1 COS domain.
    Contains 1 fibronectin type-III domain.
    Contains 1 RING-type zinc finger.
  • 翻译后修饰
    Phosphorylated on serine and threonine residues.
  • 细胞定位
    Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BBBG 1 antibody
    • BBBG1 antibody
    • E3 ubiquitin-protein ligase Midline-1 antibody
    • Finger on X and Y mouse homolog of antibody
    • FXY antibody
    • GBBB 1 antibody
    • GBBB1 antibody
    • MID 1 antibody
    • Mid1 antibody
    • Midin antibody
    • Midline 1 (Opitz/BBB syndrome) antibody
    • Midline 1 antibody
    • Midline 1 ring finger antibody
    • Midline 1 RING finger protein antibody
    • Midline-1 antibody
    • Midline1 antibody
    • OGS 1 antibody
    • OGS1 antibody
    • OS antibody
    • OSX antibody
    • Putative transcription factor XPRF antibody
    • RING finger protein 59 antibody
    • RING finger protein Midline-1 antibody
    • RNF 59 antibody
    • RNF59 antibody
    • TRI18 antibody
    • TRI18_HUMAN antibody
    • TRIM 18 antibody
    • TRIM18 antibody
    • Tripartite motif containing protein 18 antibody
    • Tripartite motif protein TRIM18 antibody
    • Tripartite motif-containing protein 18 antibody
    • XPRF antibody
    • zinc finger on X and Y, mouse, homolog of antibody
    • Zinc finger X and Y antibody
    • ZNFXY antibody
    see all

图片

  • All lanes : Anti-MID1 antibody (ab70770) at 1/500 dilution

    Lane 1 : 293 extract
    Lane 2 : 293 extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 75 kDa
    Observed band size : 75 kDa
  • ab70770 at 1/500 dilution staining MID1 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • ab70770 at 1/50 dilution staining MID1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

文献

This product has been referenced in:
  • Dierssen M  et al. Reduced Mid1 Expression and Delayed Neuromotor Development in daDREAM Transgenic Mice. Front Mol Neurosci 5:58 (2012). WB ; Mouse . Read more (PubMed: 22563308) »
  • Li T  et al. Microcystin-LR (MCLR) Induces a Compensation of PP2A Activity Mediated by a4 Protein in HEK293 Cells. Int J Biol Sci 7:740-52 (2011). WB ; Human . Read more (PubMed: 21698000) »

See all 2 Publications for this product

客户评价及客户问答

DISCOUNT CODE: ************ Expiration date: DD MM YYYY Thank you for your interest in ab70770. The homology between the immunogen (aa 91-130 of human MID1 Uniprot ref O15344) and the Gallus gallus MID1 (Uniprot ref Q90WD1) is 87.5%. See attachme...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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