概述

  • 产品名称Anti-MID1抗体
    参阅全部 MID1 一抗
  • 描述
    兔多克隆抗体to MID1
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of human MID1

  • 阳性对照
    • 293 cell extracts, human brain tissue and HeLa cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab70770 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab70770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能May have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation.
  • 组织特异性In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain.
  • 疾病相关Defects in MID1 are the cause of Opitz syndrome type I (OS-I) [MIM:300000]. OS-I is an X-linked recessive disorder characterized by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, lip-palate-laryngotracheal clefts, imperforate anus, developmental delay and congenital heart defects. OS-I mutations produce proteins with a decreased affinity for microtubules.
  • 序列相似性Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 B30.2/SPRY domain.
    Contains 1 COS domain.
    Contains 1 fibronectin type-III domain.
    Contains 1 RING-type zinc finger.
  • 翻译后修饰Phosphorylated on serine and threonine residues.
  • 细胞定位Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BBBG 1 antibody
    • BBBG1 antibody
    • E3 ubiquitin-protein ligase Midline-1 antibody
    • Finger on X and Y mouse homolog of antibody
    • FXY antibody
    • GBBB 1 antibody
    • GBBB1 antibody
    • MID 1 antibody
    • Mid1 antibody
    • Midin antibody
    • Midline 1 (Opitz/BBB syndrome) antibody
    • Midline 1 antibody
    • Midline 1 ring finger antibody
    • Midline 1 RING finger protein antibody
    • Midline-1 antibody
    • Midline1 antibody
    • OGS 1 antibody
    • OGS1 antibody
    • OS antibody
    • OSX antibody
    • Putative transcription factor XPRF antibody
    • RING finger protein 59 antibody
    • RING finger protein Midline-1 antibody
    • RNF 59 antibody
    • RNF59 antibody
    • TRI18 antibody
    • TRI18_HUMAN antibody
    • TRIM 18 antibody
    • TRIM18 antibody
    • Tripartite motif containing protein 18 antibody
    • Tripartite motif protein TRIM18 antibody
    • Tripartite motif-containing protein 18 antibody
    • XPRF antibody
    • zinc finger on X and Y, mouse, homolog of antibody
    • Zinc finger X and Y antibody
    • ZNFXY antibody
    see all

Anti-MID1 antibody 图像

  • All lanes : Anti-MID1 antibody (ab70770) at 1/500 dilution

    Lane 1 : 293 extract
    Lane 2 : 293 extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 75 kDa
    Observed band size : 75 kDa
  • ab70770 at 1/500 dilution staining MID1 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • ab70770 at 1/50 dilution staining MID1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

Anti-MID1 antibody (ab70770)参考文献

This product has been referenced in:
  • Dierssen M  et al. Reduced Mid1 Expression and Delayed Neuromotor Development in daDREAM Transgenic Mice. Front Mol Neurosci 5:58 (2012). WB ; Mouse . Read more (PubMed: 22563308) »
  • Li T  et al. Microcystin-LR (MCLR) Induces a Compensation of PP2A Activity Mediated by a4 Protein in HEK293 Cells. Int J Biol Sci 7:740-52 (2011). WB ; Human . Read more (PubMed: 21698000) »

See all 2 Publications for this product

Product Wall

DISCOUNT CODE: ************ Expiration date: DD MM YYYY Thank you for your interest in ab70770. The homology between the immunogen (aa 91-130 of human MID1 Uniprot ref O15344) and the Gallus gallus MID1 (Uniprot ref Q90WD1) is 87.5%. See attachme...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"