概述

  • 产品名称Anti-MIA40抗体
    参阅全部 MIA40 一抗
  • 描述
    兔多克隆抗体to MIA40
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N-terminal amino acids 2 - 51 (SYCRQEGKDR IIFVTKEDHE TPSSAELVAD DPNDPYEEHG LILPNGNINW) of Human MIA40 (NP_001091972)

  • 阳性对照
    • Lysate from MIA40-transfected 293T cells

性能

应用

Our Abpromise guarantee covers the use of ab87033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Probably acts by forming a redox cycle with GFER/ERV1 that involves a disulfide relay system. Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS.
  • 组织特异性Expressed in all tissues tested, suggesting an ubiquitous expression.
  • 序列相似性Contains 1 CHCH domain.
  • 结构域The CHCH domain contains a conserved twin Cys-X(9)-Cys motif which is required for import and stability of MIA40 in mitochondria.
  • 翻译后修饰Forms intrachain disulfide bridges, but exists in different redox states.
  • 细胞定位Mitochondrion intermembrane space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CHCHD4 antibody
    • Coiled coil helix coiled coil helix domain containing 4 antibody
    • Coiled coil helix coiled coil helix domain containing protein 4 antibody
    • Coiled-coil-helix-coiled-coil-helix domain-containing protein 4 antibody
    • FLJ31709 antibody
    • MIA40_HUMAN antibody
    • Mitochondrial intermembrane space import and assembly 40 homolog antibody
    • Mitochondrial intermembrane space import and assembly protein 40 antibody
    • TIMM40 antibody
    • Translocase of inner mitochondrial membrane 40 homolog antibody
    see all

Anti-MIA40 antibody 图像

  • Anti-MIA40 antibody (ab87033) at 1 µg/ml + lysate from MIA40-transfected 293T cells at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 16 kDa

Anti-MIA40 antibody (ab87033)参考文献

This product has been referenced in:
  • Zhuang J  et al. Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity. Proc Natl Acad Sci U S A 110:17356-61 (2013). ICC/IF . Read more (PubMed: 24101517) »
  • Vascotto C  et al. Knock-in reconstitution studies reveal an unexpected role of Cys-65 in regulating APE1/Ref-1 subcellular trafficking and function. Mol Biol Cell 22:3887-3901 (2011). ICC/IF ; Human . Read more (PubMed: 21865600) »

See all 2 Publications for this product

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