概述

  • 产品名称Anti-MIA3抗体
  • 描述
    兔多克隆抗体to MIA3
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 1 and 120 of MIA3 (XP_496436).

  • 阳性对照
    • A431, H1299, HeLa and HepG2 cell lines

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab97881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 214 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 功能Required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. May participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1.
  • 组织特异性Broadly expressed, except in bone marrow and peripheral blood mononuclear cells. Down-regulated in melanoma tissue.
  • 序列相似性Belongs to the MIA/OTOR family. Tango1 subfamily.
    Contains 1 SH3 domain.
  • 结构域The proline-rich region (PRD) mediates the interaction with COPII coat subunits Sec23/24.
    Although 2 transmembrane domains are predicted, PubMed:19269366 showed that it only contains one transmembrane domain. The other predicted transmembrane region is probably a hairpin-type region embedded into the membrane, which does not cross the membrane. It is unclear which of the 2 predicted transmembrane regions is the transmembrane or the hairpin-type region.
  • 细胞定位Endoplasmic reticulum membrane. Localizes at endoplasmic reticulum exit sites. After loading of COL7A1 into transport carriers, it is not incorporated into COPII carriers and remains in the endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARNT antibody
    • C219 reactive peptide antibody
    • C219-reactive peptide antibody
    • D320 antibody
    • Melanoma inhibitory activity family, member 3 antibody
    • Melanoma inhibitory activity protein 3 antibody
    • Mia3 antibody
    • MIA3_HUMAN antibody
    • RP11-378J18.4 antibody
    • TANGO antibody
    • Transport and Golgi organization protein 1 antibody
    • UNQ6077 antibody
    see all

Anti-MIA3 antibody 图像

  • All lanes : Anti-MIA3 antibody (ab97881) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : Hep G2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 214 kDa

    5% SDS PAGE.

  • Immunofluorescence analysis of methanol fixed HepG2 cells, using ab97881 at a 1/200 dilution.
    Lower image: costained with Hoechst 33342.

Anti-MIA3 antibody (ab97881)参考文献

ab97881 has not yet been referenced specifically in any publications.

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