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Our Abpromise guarantee covers the use of ab110613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.25 - 1 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIA2.|
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MIA2 (ab58973). Using ab58973 as the capture antibody and ab110613 as the detection antibody, the detection limit for recombinant Human MIA2 is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 62 kDa. The detection limit for recombinant Human MIA2 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.|
ab110613 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"