概述

  • 产品名称
    Anti-MHF2抗体
  • 描述
    兔多克隆抗体to MHF2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Human MHF2.

  • 阳性对照
    • This antibody gave a positive signal in Human Liver tissue lysate as well as the following human whole cell lysates: HEK293; U2OS; HepG2.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab104236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 9 kDa).

靶标

  • 功能
    DNA-binding component of the FA core complex involved in DNA damage repair and genome maintenance. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. Component of the APITD1/CENPS complex that is essential for the stable assembly of the outer kinetochore. Plays an important role in mitotic progression and chromosome segregation.
  • 序列相似性
    Belongs to the CENPX family.
  • 细胞定位
    Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CENP X antibody
    • CENP-X antibody
    • CENPX antibody
    • CENPX_HUMAN antibody
    • Centromere protein X antibody
    • FAAP10 antibody
    • FANCM interacting histone fold protein 2 antibody
    • FANCM-interacting histone fold protein 2 antibody
    • Fanconi anemia associated polypeptide of 10 kDa antibody
    • Fanconi anemia-associated polypeptide of 10 kDa antibody
    • MGC14480 antibody
    • Retinoic acid inducible gene D9 protein homolog antibody
    • Retinoic acid-inducible gene D9 protein homolog antibody
    • Stimulated by retinoic acid gene 13 protein homolog antibody
    • STRA13 antibody
    see all

图片

  • All lanes : Anti-MHF2 antibody (ab104236) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 9 kDa
    Observed band size : 11 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 160 kDa,46 kDa,51 kDa,58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

实验方案

文献

ab104236 has not yet been referenced specifically in any publications.

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