Anti-MHC Qa1b抗体[6A8.6F10.1A6] (ab72487)
Key features and details
- Mouse monoclonal [6A8.6F10.1A6] to MHC Qa1b
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse
- Isotype: IgG1
概述
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产品名称
Anti-MHC Qa1b抗体[6A8.6F10.1A6] -
描述
小鼠单克隆抗体[6A8.6F10.1A6] to MHC Qa1b -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Mouse MHC Qa1b.
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阳性对照
- ConA-stimulated Balb/c mouse splenocytes.
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常规说明
ab72487 can be used for flow cytometric assay to detect Qa1b on immuno-regulatory antigen presenting cells and activated splenic T cells on Qa1b expressing mice (e.g. BALB/c, C57BL/6) and it can also be used for functional assays to block target cell recognition by CTLs specific for Qa1 restricted antigen presentation and other functional study where Qa1b associated immuno-regulation may work.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading...
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纯度
Protein G purified -
Primary antibody说明
ab72487 can be used for flow cytometric assay to detect Qa1b on immuno-regulatory antigen presenting cells and activated splenic T cells on Qa1b expressing mice (e.g. BALB/c, C57BL/6) and it can also be used for functional assays to block target cell recognition by CTLs specific for Qa1 restricted antigen presentation and other functional study where Qa1b associated immuno-regulation may work. -
克隆
单克隆 -
克隆编号
6A8.6F10.1A6 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72487于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 0.1-0.5µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
Use a concentration of 1 - 2 µg/ml.
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说明 |
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Flow Cyt
Use 0.1-0.5µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 - 2 µg/ml. |
靶标
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相关性
Qa1b is a murine nonclassical MHC class I molecule, encoded by the T23 gene in the T region. It functions in both innate and adaptive immune responses, regulating NK cell activation and serving as a restriction element for a subpopulation of CD8+ T cells. It is expressed in a wide range of tissues similar to MHC class Ia molecules. However, Qa1b is relatively nonpolymorphic and is expressed at lower levels than MHC class Ia molecules. In contrast to classical MHC class I molecules, Qa1b predominantly binds a single nonameric peptide, Qa1b determinant modifier (Qdm), derived from the signal sequence of H-2D/L molecules. The Qa-1/Qdm complex serves as a ligand for CD94/NKG2 natural killer (NK) receptor(s) expressed on a major fraction of NK cells and CD8+ immuno-suppressive regulatory T cells. Qa-1b, expressed on immuno-regulatory B cells and other antigen presenting cells, serves for antigen-presentation to immuno-regulatory CD8+ T. Moreover, Qa-1b serves an MHC restrictive function because, unlike conventional MHC molecules, it transiently expresses on activated CD4+ T cells. Recently it has also been shown that Qa-1b is ubiquitously expressed in the immune-privileged anterior chamber of the eye. Additionally, it has been suggested that TCR specific, Qa-1b-restricted regulatory CD8+ T cells selectively down regulate antigen-activated T cells expressing TCRs of certain affinities. These regulatory CD8+ T cells may control the peripheral TCR repertoire during the course of immune responses to both self and foreign antigens. HLA-E is the functional ortholog of Qa-1b in humans. -
细胞定位
Membrane; Single-pass type I membrane protein. -
数据库链接
- Entrez Gene: 15040 Mouse
- SwissProt: P06339 Mouse
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别名
- H2 class I histocompatibility antigen D37 alpha chain antibody
- H2 Q1 antibody
- H2 T23 antibody
see all
图片
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Western blot analysis of mouse thymus tissue lysate using ab72487 at 2 µg/ml.
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Flow cytometric analysis of MHC Qa1b in unstimulated Balb/c mouse splenocytes using A) isotype control and B) ab72487 (with an anti-mouse IgG1-PE secondary) at 0.25 ug/106 cells. A shift is seen in only a small percentage of cells.
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Flow cytometric analysis of MHC Qa1b in 72 hour ConA-stimulated Balb/c mouse splenocytes using A) isotype control and B) ab72487 (with an anti-mouse IgG1-PE secondary) at 0.25 ug/106 cells. A shift is seen in the entire population of cells.
数据表及文件
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Datasheet download
文献 (2)
ab72487 被引用在 2 文献中.
- Sasaki T et al. Microenvironmental stresses induce HLA-E/Qa-1 surface expression and thereby reduce CD8(+) T-cell recognition of stressed cells. Eur J Immunol 46:929-40 (2016). PubMed: 26711740
- Gan Y et al. Ischemic neurons recruit natural killer cells that accelerate brain infarction. Proc Natl Acad Sci U S A 111:2704-9 (2014). ICC/IF ; Mouse . PubMed: 24550298