Anti-MHC class II RT Ib抗体[F17-23-2] (ab33745)

概述

  • 产品名称Anti-MHC class II RT Ib抗体[F17-23-2]
    参阅全部 MHC class II RT Ib 一抗
  • 描述
    小鼠单克隆抗体[F17-23-2] to MHC class II RT Ib
  • 特异性Recognizes the rat RT1B MHC class II antigen, reacting with haplotypes a, l and n expressed on rat strains including DA, LEW, and BN.
  • 经测试应用适用于: Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Partially purified MHC antigens (Rat)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 5-10% Foetal calf serum, PBS, 0.2M Tris HCl. pH 7.4
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号F17-23-2
  • 骨髓瘤NS1
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab33745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
IHC-Fr
  • 应用说明Flow Cyt: Use 50µl of 1/5 diluted antibody sample for 106 cells in 100µl.
    IHC-Fr: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Recognizes a polymorphic determinant on rat Ia present on Lewis, Wistar, and AO rat strains (although it is apparently expressed in lower amounts in AO rats) but not BN, DA, or PVG/c strains. Also cross-reacts with mouse strains (MHC haplotypes b and s ) and analysis of recombinant mouse strains shows that the determinant maps to the I-A region and correlates with mouse Ia specificity 9 (1). Ia antigens are recognized on approximately 18% of thymocytes but does not significantly label the majority of peripheral T lymphocytes (1,3,4). Thus, is particularly useful for distinguishing Ia positive cells from different rat strains and has been used in the recognition of cells of donor origin in bone marrow reconstituted radiation chimeras.
    • 细胞定位Type I membrane protein.
    • 别名
      • major histocompatibility complex antibody
      • major histocompatibility complex class II antibody

    Anti-MHC class II RT Ib antibody [F17-23-2] (ab33745)参考文献

    This product has been referenced in:
    • Mirenda V  et al. Modified dendritic cells coexpressing self and allogeneic major histocompatability complex molecules: an efficient way to induce indirect pathway regulation. J Am Soc Nephrol 15:987-97 (2004). Read more (PubMed: 15034101) »

    See 1 Publication for this product

    Product Wall

    Merci de votre appel de toutà l'heure.

    Voici les résultats du blast promises. Comme discuté au téléphone, nous avons aussi des anticorps contre le RT1 class Ib de rat directement, comme par exemple le ab3374...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"