FITC荧光Anti-H2-Ea抗体[M5/114.15.2] (ab93561)
Key features and details
- FITC Rat monoclonal [M5/114.15.2] to H2-Ea
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
概述
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产品名称
FITC荧光Anti-H2-Ea抗体[M5/114.15.2]
参阅全部 H2-Ea 一抗 -
描述
FITC荧光大鼠单克隆抗体[M5/114.15.2] to H2-Ea -
宿主
Rat -
偶联物
FITC. Ex: 493nm, Em: 528nm -
特异性
ab93561 reacts with the mouse MHC Class II, both I-A and I-E subregion-encoded glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u but not from mice carrying the H-2s or H-2f haplotypes. -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse H2-Ea.
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阳性对照
- Flow cyt: Mouse C57BI/6 splenocytes
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常规说明
The M5/114.15.2 antibody is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.16% Sodium phosphate, 0.1% Gelatin -
Concentration information loading...
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纯度
Protein G purified -
Primary antibody说明
The M5/114.15.2 antibody is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes. -
克隆
单克隆 -
克隆编号
M5/114.15.2 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab93561于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use at an assay dependent concentration.
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说明 |
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Flow Cyt
Use at an assay dependent concentration. |
靶标
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相关性
The MHC Class II IA + IE molcules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 b, d, q, r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. -
细胞定位
Single-pass type I membrane protein -
数据库链接
- Entrez Gene: 111861 Mouse
- SwissProt: P01904 Mouse
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别名
- AI323765 antibody
- E alpha f antibody
- H 2Ea antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab93561 被引用在 9 文献中.
- Zhang S et al. Salmonella infection leads to severe intestinal inflammation and increased CD4+FoxP3+ Treg cells in streptozotocin-induced hyperglycemic mice. Mol Med Rep 19:5377-5385 (2019). PubMed: 31059096
- Meng Y et al. Paeonol ameliorates imiquimod-induced psoriasis-like skin lesions in BALB/c mice by inhibiting the maturation and activation of dendritic cells. Int J Mol Med 39:1101-1110 (2017). PubMed: 28339016
- Zeng SL et al. Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function. Mol Med Rep 12:2511-20 (2015). PubMed: 25936350
- Unternaehrer JJ et al. The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A 104:234-9 (2007). PubMed: 17190803
- Gueirard P et al. Bordetella bronchiseptica persists in the nasal cavities of mice and triggers early delivery of dendritic cells in the lymph nodes draining the lower and upper respiratory tract. Infect Immun 71:4137-43 (2003). PubMed: 12819105
- Bagavant H et al. Induction and immunohistology of autoimmune ovarian disease in cynomolgus macaques (Macaca fascicularis). Am J Pathol 160:141-9 (2002). PubMed: 11786408
- Li C et al. Cooperative interaction of Ig(alpha) and Ig(beta) of the BCR regulates the kinetics and specificity of antigen targeting. Int Immunol 14:1179-91 (2002). IP . PubMed: 12356683
- Mendiratta SK et al. Analysis of T-cell hybridomas with an unusual MHC class II-dependent ligand specificity. Immunology 89:238-44 (1996). PubMed: 8943720
- Germain RN et al. A single monoclonal anti-Ia antibody inhibits antigen-specific T cell proliferation controlled by distinct Ir genes mapping in different H-2 I subregions. J Immunol 128:1409-13 (1982). PubMed: 6173436