小鼠单克隆抗体[17-3-3.4] to MHC class II I E kappa (FITC)
偶联物FITC. Ex: 493nm, Em: 528nm
特异性This antibody reacts with the I-Ek class II alloantigen on cells from mice of the H-2r haplotype; reactivity with H-2d and H-2p haplotypes has not been observed. [Cells from mice of the H-2b, H-2f, H-2g7, H-2q,
and H-2s haplotypes do not express I-E antigen.]
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明Flow Cyt: Use 1µg for 106 cells.
Complement-mediated cytotoxicity: Use at an assay dependent dilution
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
相关性A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
细胞定位Type I membrane protein
H2 Ea antibody
Anti-MHC class II I E kappa antibody [17-3-3.4] (FITC) 图像
Flow Cytometry - MHC class II I E kappa antibody [17-3-3.4] (FITC) (ab25679)
AKR spleen cells were double-stained with ab25679 (at 1µg/106 cells) and Rat Anti-Mouse CD3ε-R-PE. Lymphocytes were gated using and analyzed by flow cytometry.
Anti-MHC class II I E kappa antibody [17-3-3.4] (FITC) (ab25679)参考文献
This product has been referenced in:
Ozato K et al. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol124:533-40 (1980).
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