Anti-MHC class II I E kappa抗体[17-3-3.4] (FITC) (ab25679)

概述

  • 产品名称Anti-MHC class II I E kappa抗体[17-3-3.4] (FITC)
    参阅全部 MHC class II I E kappa 一抗
  • 描述
    小鼠单克隆抗体[17-3-3.4] to MHC class II I E kappa (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性This antibody reacts with the I-Ek class II alloantigen on cells from mice of the H-2r haplotype; reactivity with H-2d and H-2p haplotypes has not been observed. [Cells from mice of the H-2b, H-2f, H-2g7, H-2q, and H-2s haplotypes do not express I-E antigen.]
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Tissue/ cell preparation (Mouse) -C3H mouse skin graft and splenocytes.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号17-3-3.4
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab25679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明Flow Cyt: Use 1µg for 106 cells.
    Complement-mediated cytotoxicity: Use at an assay dependent dilution


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
    • 细胞定位Type I membrane protein
    • 别名
      • H2 Ea antibody
      • H2-Ea antibody

    Anti-MHC class II I E kappa antibody [17-3-3.4] (FITC) 图像

    • AKR spleen cells were double-stained with ab25679 (at 1µg/106 cells) and Rat Anti-Mouse CD3ε-R-PE.  Lymphocytes were gated using and analyzed by flow cytometry.

    Anti-MHC class II I E kappa antibody [17-3-3.4] (FITC) (ab25679)参考文献

    This product has been referenced in:
    • Ozato K  et al. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol 124:533-40 (1980). Read more (PubMed: 7188699) »

    See 1 Publication for this product

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