小鼠单克隆抗体[14-4-4S] to MHC class II I E kappa (Phycoerythrin)
Phycoerythrin. Ex: 488nm, Em: 575nm
This antibody is specific to an epitope on mouse I-E? MHC class II alloantigen. The antibody reacts with the I-E? class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. The antibody has also been reported to crossreact with the rat class II alloantigen RT1D.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 0.2µg for 106 cells.
IHC-Fr(cetone fixed): Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
BL: Use at an assay dependent dilution for in vitro blocking of antigen presentation.
Complement dependent cytotoxicity: Use at an assay dependent dilution.
In vitro induction of antigen specific B cell differentiation: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
Farr A et al. Differential expression of CLIP:MHC class II and conventional endogenous peptide:MHC class II complexes by thymic epithelial cells and peripheral antigen-presenting cells. Eur J Immunol26:3185-93 (1996).
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Roy M et al. Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses. Eur J Immunol25:596-603 (1995).
Read more (PubMed: 7533092) »