Anti-MHC class II I E kappa抗体[14-4-4S] (FITC) (ab25023)

概述

  • 产品名称
    Anti-MHC class II I E kappa抗体[14-4-4S] (FITC)
    参阅全部 MHC class II I E kappa 一抗
  • 描述
    小鼠单克隆抗体[14-4-4S] to MHC class II I E kappa (FITC)
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm
  • 特异性
    ab25023 recognises MHC class II I E kappa alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. It cross reacts with alloantigen RT1D. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express this antigen.
  • 经测试应用
    适用于: Flow Cyt, IP, Blocking, IHC-Fr, Functional Studiesmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Tissue/ cell preparation: C3H mouse skin graft and splenocytes.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 克隆
    单克隆
  • 克隆编号
    14-4-4S
  • 同种型
    IgG2a
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab25023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
IP
Blocking
IHC-Fr
Functional Studies
  • 应用说明
    BL: Use at an assay dependent dilution for in vitro blocking of antigen presentation.
    Flow Cyt: Use 1µg for 106 cells.
    FuncS: Use at an assay dependent dilution for complement dependent cytotoxicity studies and in vitro induction of antigen specific B cell differentiation.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
    • 细胞定位
      Type I membrane protein
    • 别名
      • H2 Ea antibody
      • H2-Ea antibody

    图片

    • Flow cytometry analysis staining MHC class II I E kappa in mouse splenocytes using ab25023 at a dilution of 1 ug/106 cells.

    文献

    ab25023 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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