Anti-MHC class II I E kappa抗体[14-4-4S] (FITC) (ab25023)

概述

  • 产品名称Anti-MHC class II I E kappa抗体[14-4-4S] (FITC)
    参阅全部 MHC class II I E kappa 一抗
  • 描述
    小鼠单克隆抗体[14-4-4S] to MHC class II I E kappa (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性ab25023 recognises MHC class II I E kappa alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. It cross reacts with alloantigen RT1D. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express this antigen.
  • 经测试应用适用于: Flow Cyt, IP, Blocking, IHC-Fr, Functional Studiesmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Tissue/ cell preparation: C3H mouse skin graft and splenocytes.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号14-4-4S
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab25023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
IP
Blocking
IHC-Fr
Functional Studies
  • 应用说明BL: Use at an assay dependent dilution for in vitro blocking of antigen presentation.
    Flow Cyt: Use 1µg for 106 cells.
    FuncS: Use at an assay dependent dilution for complement dependent cytotoxicity studies and in vitro induction of antigen specific B cell differentiation.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
    • 细胞定位Type I membrane protein
    • 别名
      • H2 Ea antibody
      • H2-Ea antibody

    Anti-MHC class II I E kappa antibody [14-4-4S] (FITC) 图像

    • Flow cytometry analysis staining MHC class II I E kappa in mouse splenocytes using ab25023 at a dilution of 1 ug/106 cells.

    Anti-MHC class II I E kappa antibody [14-4-4S] (FITC) (ab25023)参考文献

    ab25023 has not yet been referenced specifically in any publications.

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