The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ab18454 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
Spencer JS et al. Expression and function of mixed isotype MHC class II molecules in normal mice. J Immunol151:6822-32 (1993).
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Pruitt SK et al. The effect of xenoreactive antibody and B cell depletion on hyperacute rejection of guinea pig-to-rat cardiac xenografts. Transplantation56:1318-24 (1993).
Read more (PubMed: 8278996) »