Anti-MHC class II I E kappa抗体[14-4-4S] (ab25681)

概述

  • 产品名称Anti-MHC class II I E kappa抗体[14-4-4S]
    参阅全部 MHC class II I E kappa 一抗
  • 描述
    小鼠单克隆抗体[14-4-4S] to MHC class II I E kappa
  • 特异性This antibody is specific to an epitope on mouse I-E? MHC class II alloantigen. The antibody reacts with the I-E? class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. The antibody has also been reported to cross react with the rat class II alloantigen RT1D.
  • 经测试应用适用于: Functional Studies, Flow Cyt, IP, IHC-Fr, Blockingmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Tissue/ cell preparation : C3H mouse skin graft and splenocytes.

  • 常规说明


性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 8.20
    Constituent: 100% Borate buffered saline

    No preservatives or amine-containing buffer salts added.
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号14-4-4S
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领���

应用

Our Abpromise guarantee covers the use of ab25681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

In vitro induction of antigen specific B cell differentiation. Complement dependent cytotoxicity.

Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Acetone fixed.

Blocking Use at an assay dependent concentration.

In vitro blocking of antigen presentation.

靶标

  • 相关性A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
  • 细胞定位Type I membrane protein
  • 别名
    • H2 Ea antibody
    • H2-Ea antibody

Anti-MHC class II I E kappa antibody [14-4-4S] (ab25681)参考文献

This product has been referenced in:
  • Farr A  et al. Differential expression of CLIP:MHC class II and conventional endogenous peptide:MHC class II complexes by thymic epithelial cells and peripheral antigen-presenting cells. Eur J Immunol 26:3185-93 (1996). Read more (PubMed: 8977321) »
  • Roy M  et al. Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses. Eur J Immunol 25:596-603 (1995). Read more (PubMed: 7533092) »

See all 5 Publications for this product

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