Anti-MHC Class II抗体[NIMR-4] (ab25333)


  • 产品名称Anti-MHC Class II抗体[NIMR-4]
    参阅全部 MHC Class II 一抗
  • 描述
    大鼠单克隆抗体[NIMR-4] to MHC Class II
  • 特异性ab25333 reacts specifically with a non-polymorphic I-A-encoded epitope on MHC Class II antigens.
  • 经测试应用适用于: Flow Cyt, ICC/IF, IHC-P, Functional Studiesmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 阳性对照
    • ICC/IF: Raw264.7 cells.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号NIMR-4
  • 同种型IgG2b
  • 轻链类型kappa
  • 研究领域


Our Abpromise guarantee covers the use of ab25333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µl for 106 cells.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
IHC-P 1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Functional Studies Use at an assay dependent concentration.

Complement-dependent cytotoxicity assays.


  • 功能Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • 序列相似性Belongs to the MHC class II family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 细胞定位Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D6S221E antibody
    • DMA antibody
    • DMB antibody
    • DP beta 1 antibody
    • DP beta 1 chain antibody
    • DP(W4) beta chain antibody
    • DPB 1 antibody
    • DPB1 antibody
    • DPB1_HUMAN antibody
    • DRB antibody
    • H2Ea antibody
    • HLA class II histocompatibility antigen antibody
    • HLA class II histocompatibility antigen DM beta chain antibody
    • HLA DMB antibody
    • HLA DPB1 antibody
    • HLA-DP antibody
    • HLA-DP1B antibody
    • HLA-DPB antibody
    • HLA-DPB1 antibody
    • HLADM antibody
    • HLADP1B antibody
    • Major histocompatibility complex class II antibody
    • Major histocompatibility complex class II DP beta 1 antibody
    • MHC class II antigen DMB antibody
    • MHC class II antigen DPB1 antibody
    • MHC DPB1 antibody
    • RING6 antibody
    • RING7 antibody
    see all

Anti-MHC Class II antibody [NIMR-4] 图像

  • ab25333 staining MHC Class II in mouse spleen, white pulp tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Tween-20 in PBS and blocked with 1% BSA for 40 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer pH 6. Samples were incubated with primary antibody (1/150 in TBS + 1% BSA) for 18 hours at 4°C. A Biotin-conjugated goat anti-rat IgG polyclonal (1/100) was used as the secondary antibody.

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  • ICC/IF image of ab25333 stained Raw264.7 cells. The cells were 4% Formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25333, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab98420, DyLight® 488 Goat anti-Rat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MHC Class II antibody [NIMR-4] (ab25333)参考文献

ab25333 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Sample Mouse Tissue sections (spleen, white pulp)
Specification spleen, white pulp
Permeabilization Yes - 0,1% Tween-20 in TBS
Fixative Paraformaldehyde

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提交于 Aug 01 2014

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Unfortunately, none of our MHC class II antibodies are raised against specific haplotypes. The validation for ab25333 was performed using BALB/c spleen cells, and we do not currently have any feedback from custome...

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