Anti-MHC Class II抗体[MRC OX-6] (ab23990)

概述

  • 产品名称Anti-MHC Class II抗体[MRC OX-6]
    参阅全部 MHC Class II 一抗
  • 描述
    小鼠单克隆抗体[MRC OX-6] to MHC Class II
  • 特异性This antibody recognises a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. The antibody cross-reacts with certain mouse strains of MHC haplotypes k and s and analysis of recombinant strains showed that the determinants mapped to the I-A region of the mouse MHC.
  • 经测试应用适用于: WB, ICC, IHC-P, ICC/IF, Flow Cyt, IHC-Fr, IHC (PFA fixed)more details
  • 种属反应性
    与反应: Rat
    预测可用于: Mouse
  • 免疫原

    Thymocyte membrane glycoprotein (Rat).

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Rat normal spleen.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆单克隆
  • 克隆编号MRC OX-6
  • 骨髓瘤NS1
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab23990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
IHC (PFA fixed) 1/100.

靶标

  • 功能Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • 序列相似性Belongs to the MHC class II family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 细胞定位Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D6S221E antibody
    • DMA antibody
    • DMB antibody
    • DP beta 1 antibody
    • DP beta 1 chain antibody
    • DP(W4) beta chain antibody
    • DPB 1 antibody
    • DPB1 antibody
    • DPB1_HUMAN antibody
    • DRB antibody
    • H2Ea antibody
    • HLA class II histocompatibility antigen antibody
    • HLA class II histocompatibility antigen DM beta chain antibody
    • HLA class II histocompatibility antigen, DP beta 1 chain antibody
    • HLA class II histocompatibility antigen, DP(W4) beta chain antibody
    • HLA DMB antibody
    • HLA DP1A antibody
    • HLA DPB1 antibody
    • HLA SB alpha chain antibody
    • HLA-A antibody
    • HLA-A histocompatibility type antibody
    • HLA-DP antibody
    • HLA-DP histocompatibility type, beta-1 subunit antibody
    • HLA-DP1B antibody
    • HLA-DPB antibody
    • HLA-DPB1 antibody
    • HLADM antibody
    • HLADP1B antibody
    • HLASB antibody
    • HLASB histocompatibility type antibody
    • Human MHC class II HLA SB alpha antibody
    • LA class II histocompatibility antigen DP alpha 1 chain antibody
    • Major histocompatibility complex class II antibody
    • Major histocompatibility complex class II DP alpha 1 antibody
    • Major histocompatibility complex class II DP beta 1 antibody
    • Major histocompatibility complex, class I, A antibody
    • MHC class II antigen DMB antibody
    • MHC class II antigen DPB1 antibody
    • MHC class II DP3 alpha antibody
    • MHC class II DPA1 antibody
    • MHC class II HLA-DP-beta-1 antibody
    • MHC DPB1 antibody
    • MHC HLA DPB1 antibody
    • PLT1 antibody
    • Primed lymphocyte test 1 antibody
    • RING6 antibody
    • RING7 antibody
    see all

Anti-MHC Class II antibody [MRC OX-6] 图像

  • Immunohistochemistical detection of MHC Class II using antibody ab23990 on PFA-fixed rat spleen tissue sections. Antibody diluted at 1/100 and incubated for2 hours in  TBS/BSA/Tween/azide. Secondary antibody: anti mouse IgG conjugated to biotin (1/100). After dissection of spleen from PFA-perfused specimen it was sampled and further immersion-fixed for two Hrs. After subsequent immersion in 30% Sucrose, specimens were snap-frozen. Before immunostaining, the 8 micron sections were placed in a 60 degree C oven for 60 mins to enhance adhesion. The submitted image shows white pulp (PALS) and a small area of red pulp (RP-upper left). The Periarteriolar Sheath (PALS) with it's Central Arteriole/artery (CA)shows many positive cells (B-lymphocytes and Macrophages) and negative lymphocytes (T-cells?). The Marginal Sinus (MS) is clearly seen between the PALS and the Marginal Zone (MZ). There is a clear Germinal Centre (GS) in this image. Lymphoid tissue is not my

    See Abreview

  • ab23990 staining MHC Class II in Rat spleen tissue by Immunohistochemistry (Frozen sections). The sections were fixed in PFA prior to blocking with 10% serum for 20 minutes at 24°C. The primary antibody was diluted 1/100 in PBS and incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-mouse polyclonal was used as the secondary antibody, diluted 1/1000 for 1 hour at room temperature. Counterstained with Hoechst 33258 (blue) for 10 minutes at room temperature

    See Abreview

  • IHC image of MHC Class II staining in Rat normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23990, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-MHC Class II antibody [MRC OX-6] (ab23990)参考文献

This product has been referenced in:
  • Feng M  et al. ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages. Poult Sci N/A:N/A (2016). Flow Cyt . Read more (PubMed: 27486255) »
  • Klyachkin YM  et al. Pharmacological Elevation of Circulating Bioactive Phosphosphingolipids Enhances Myocardial Recovery After Acute Infarction. Stem Cells Transl Med 4:1333-43 (2015). Read more (PubMed: 26371341) »

See all 11 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Brain, Hypothalamus)
Permeabilization Yes - 0.1% Tween-20 in PBS
Specification Brain, Hypothalamus
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Ms. Chia-Li Lin

Verified customer

提交于 Nov 19 2015

Thank you for contacting us.

We do sell this specific clone to MHC II. The product number for this antibody is ab23990. It is sold at 125ug size at a concentration of 1mg/ml. These are currently in stock both in the US and at our other off...

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Thank you for your enquiry.

I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any antibody tested in the specie...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Rat Cultured Cells (33B)
Specification 33B
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Jul 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (B50 Neuroblastoma Cell line)
Specification B50 Neuroblastoma Cell line
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Jun 21 2012

Thanks again for your call last week amd for your question about this antibody. I sincerely apologize but I just received further information from the lab, and the last email I sent you contained misinformation. This antibody has actually been tested i...

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Thank you for your call yesterday and for your patience while I have been in touch with the lab. I have attached a document that shows what strains have been tested with ab23990. According to the lab, we have not tested C57/Bl6, however every strain te...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Spleen)
Specification Spleen
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Sep 23 2011

Thank you for your patience. I have received the following explanation from the lab that will hopefully help to answer your questions: Please see below for a breakdown of rat and mouse MHC molecules. Rat MHC Class II molecules are RT1.B, RT1....

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Thank you for your enquiry. Permeabilisation is normally required for intracellular Flow Cytometry as this involves whole cells. However, for immunohistochemistry (whether using fluorescence or HRP), the cells have already been sliced, so further p...

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