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Recombinant full length protein, corresponding to amino acids 1-259 of Human MHC Class II beta chain HLA-DPB1
Our Abpromise guarantee covers the use of ab55152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
|Flow Cyt||Use 0.5µg for 106 cells. (Also see PMID: 18941249)
ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ab55152 staining MHC Class II in Human bowel tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was enzymatic. Samples were incubated with primary antibody (1/50 in milk) for 21 hours at 4°C. A Biotin-conjugated Rabbit anti-mouse polyclonal (1/300) was used as the secondary antibody.
ICC/IF image of ab55152 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab55152 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.