Anti-MHC Class I H2 Kd抗体[34-1-2S] (ab25334)


  • 产品名称Anti-MHC Class I H2 Kd抗体[34-1-2S]
    参阅全部 MHC Class I H2 Kd 一抗
  • 描述
    小鼠单克隆抗体[34-1-2S] to MHC Class I H2 Kd
  • 特异性ab25334 binds to a common determinant in the a3 domains of H-2Kd and H-2Dd in the presence or absence of ß2 microglobulin. It cross reacts with the a3 domain of H-2Kb.
  • 经测试应用适用于: Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    C3H.SW splenocytes (Mouse)


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号34-1-2S
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域


Our Abpromise guarantee covers the use of ab25334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明Flow Cyt: Use 1µg for 106 cells.
    IHC-Fr: Use at an assay dependent dilution.
    Complement-mediated cytotoxicity: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性MHC Class I H2 Kd is involved in the presentation of foreign antigens to the immune system. The “classical” MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant Beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.
    • 细胞定位Cell Membrane
    • 数据库链接
    • 别名
      • class I major histocompatibility antigen H 2Kd antibody
      • H 2 class I histocompatibility antigen K D alpha chain [Precursor] antibody
      • H 2K antibody
      • H2 K1 antibody
      • MHC class I H2 K b alpha 2 cell surface glycoprotein antibody
      • MHC class I heavy chain H2 K antibody
      • MHC H2 K transplantation antigen antibody
      • MHC K bm6 transplantation antigen antibody
      see all

    Anti-MHC Class I H2 Kd antibody [34-1-2S] (ab25334)参考文献

    This product has been referenced in:
    • Allen H  et al. Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db. Proc Natl Acad Sci U S A 83:7447-51 (1986). Read more (PubMed: 3532114) »
    • Allen H  et al. Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition sites. Nature 309:279-81 (1984). Read more (PubMed: 6201750) »

    See all 4 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (RAW264.7 cell line)
    Permeabilization No
    Specification RAW264.7 cell line
    Fixative acetone/methanol 50/50

    Dr. Julian Bursztyka

    Verified customer

    提交于 Nov 13 2015

    Application Immunohistochemistry (Frozen sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample Mouse Tissue sections (Brain section)
    Specification Brain section
    Permeabilization Yes - 0.1M PBS with 3% TritonX100
    Fixative Paraformaldehyde

    Dr. Ruma Raha-Chowdhury

    Verified customer

    提交于 Nov 05 2014

    I am very pleased to hear you would like to accept our offer and test ab25334 in IHC-P. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code...

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