Anti-MHC class I抗体[OX-18] (FITC) (ab22367)

概述

  • 产品名称Anti-MHC class I抗体[OX-18] (FITC)
    参阅全部 MHC class I 一抗
  • 描述
    小鼠单克隆抗体[OX-18] to MHC class I (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性ab22367 recognises a monomorphic determinant of rat MHC Class I (RT-1A), however quantitative measurements suggest that not all of the Class I molecules are recognised.
  • 经测试应用适用于: IHC-P, IHC-R, ELISA, Flow Cyt, IHC-Fr, ICC/IF, Othermore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Full length protein: Rat spleen cell glycoprotein

  • 阳性对照
    • Flow cytometry: rat splenocytes

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号OX-18
  • 骨髓瘤P3x63-Ag8.653
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab22367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
IHC-R Use at an assay dependent concentration. Note for IHC: Acetone fixation recommended. The antigen is sensitive to fixation with paraformaldehyde.
AP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells.

Use 10µl of suggested working dilution (neat - 1/10) for 106 cells in 100µl.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/20.
Other Use at an assay dependent concentration.

靶标

  • 相关性MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
  • 细胞定位Cell Membrane; Type I membrane protein.
  • 别名
    • A 28 antibody
    • A 9 antibody
    • Antigen presenting molecule antibody
    • Aw 24 antibody
    • Aw 68 antibody
    • CLASS I HISTOCOMPATIBILITY ANTIGEN antibody
    • H2 K1 antibody
    • H2K antibody
    • HLA A antibody
    • HLA class I histocompatibility antigen A 1 alpha chain antibody
    • HLA class I histocompatibility antigen A 11 alpha chain antibody
    • HLA class I histocompatibility antigen A 2 alpha chain antibody
    • HLA class I histocompatibility antigen A 24 alpha chain antibody
    • HLA class I histocompatibility antigen A 3 alpha chain antibody
    • HLA class I histocompatibility antigen A 30 alpha chain antibody
    • HLA class I histocompatibility antigen A 32 alpha chain antibody
    • HLA class I histocompatibility antigen A 68 alpha chain antibody
    • HLAA antibody
    • Leukocyte antigen class I A antibody
    • Major histocompatibility complex class I A antibody
    • MHC class I antigen A 1 antibody
    • MHC class I antigen A*11 antibody
    • MHC class I antigen A*2 antibody
    • MHC class I antigen A*24 antibody
    • MHC class I antigen A*3 antibody
    • MHC class I antigen A*30 antibody
    • MHC class I antigen A*32 antibody
    • MHC class I antigen A*68 antibody
    • MHC class I antigen HLA A heavy chain antibody
    • MHC class I heavy chain H2 K antibody
    see all

Anti-MHC class I antibody [OX-18] (FITC) (ab22367)参考文献

This product has been referenced in:
  • Sjöqvist S  et al. Experimental orthotopic transplantation of a tissue-engineered oesophagus in rats. Nat Commun 5:3562 (2014). IHC-Fr ; Rat . Read more (PubMed: 24736316) »
  • Akeno N  et al. IFN-{alpha} Mediates the Development of Autoimmunity both by Direct Tissue Toxicity and through Immune Cell Recruitment Mechanisms. J Immunol 186:4693-706 (2011). Flow Cyt ; Rat . Read more (PubMed: 21402899) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Pancreatic acinar cells)
Specification Pancreatic acinar cells
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Dec 08 2011

Thank you for your patience in this matter. The originator of ab22367 (MHC class I antibody [OX-18] (FITC)) has provided the following information: OX-18 is listed as being against a monomorphic determinant because it reacted with all rat str...

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Thank you for your enquiry. The antibody has not been tested on rat testis cells therefore we cannot recommend a specific amount of cells to use for this tissue. We know that the antibody worked well on rat splenocytes when 10ul of the suggested wor...

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Thank you for your enquiry. ab22367 recognizes rat MHC 1 molecules, which is generally known to be expressed on virtually all somatic, nucleated cells. We could not recognise "dlsatic fibers", so we are not really able to comment on whether we would...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"