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The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab24881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab106163 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
ab24881 staining MHC class I antibody in chicken peripheral blood mononuclear cells (PBMC), purified by density gradient centrifugation in a PBS - FBS 10% - NaN3 0.1% buffer. The sample was incubated with the primary antibody for 30 minutes at 4°C. A A secondary was not used.
Gating Strategy: SS vs FS.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"