Anti-MHC Class 1 H2 Db抗体[28-14-8] (Phycoerythrin) (ab25588)

概述

  • 产品名称Anti-MHC Class 1 H2 Db抗体[28-14-8] (Phycoerythrin)
    参阅全部 MHC Class 1 H2 Db 一抗
  • 描述
    小鼠单克隆抗体[28-14-8] to MHC Class 1 H2 Db (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特异性This antibody binds to the a3 domain of H-2Db in the presence or absence of ß2 microglobulin. It cross reacts with the a3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq.
  • 经测试应用适用于: Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    C3H.SW mouse splenocytes.

  • 表位This antibody recognises an epitope in the a3 domain of H-2Db.
  • 常规说明


    This antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells. It has been shown to be useful in studies of complement mediated cytotoxicity.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS and stabilizing agent.
  • Concentration information loading...
  • 纯度IgG fraction
  • Primary antibody说明This antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells. It has been shown to be useful in studies of complement mediated cytotoxicity.
  • 克隆单克隆
  • 克隆编号28-14-8
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab25588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

靶标

  • 相关性In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
  • 细胞定位Cell Membrane; single pass type I membrane protein
  • 数据库链接
  • 别名
    • H 2D cell surface glycoprotein antibody
    • H2 class I histocompatibility antigen, DB alpha chain antibody
    • H2D(B) antibody
    • H2D1 antibody
    • Histocompatibility 2, D region locus 1 antibody
    see all

Anti-MHC Class 1 H2 Db antibody [28-14-8] (Phycoerythrin) 图像

  • Flow cytometry analysis staining MHC Class 1 H2 Db in mouse splenocytes using ab25588 at a dilution of 0.1 μg/106 cells. Mouse IgG2a-PE was used as an isotype control.

Anti-MHC Class 1 H2 Db antibody [28-14-8] (Phycoerythrin) (ab25588)参考文献

ab25588 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. We do not have a protocol specifically for acetone fixation. However, may I recommend the excellent resource at IHC world. The following link details technical tips to consider when performing acetone fixation. http:...

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