特异性Ab25056 binds to the a3 domain of H-2Db in the presence or absence of ß2 microglobulin. It cross reacts with the a3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. Ab25056 has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 106 cells. ab18455-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
相关性In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen).
MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
细胞定位Cell Membrane; single pass type I membrane protein