Anti-MHC Class 1 H2 Db抗体[28-14-8] (ab25228)

概述

  • 产品名称Anti-MHC Class 1 H2 Db抗体[28-14-8]
    参阅全部 MHC Class 1 H2 Db 一抗
  • 描述
    小鼠单克隆抗体[28-14-8] to MHC Class 1 H2 Db
  • 特异性ab25228 binds to the alpha3 domain of H2Db in the presence or absence of beta2 microglobulin. It cross reacts with the alpha3 domain of H2Ld but not Kd or Dd, and with H-Dq and/or Lq.
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    C3H.SW splenocytes (Mouse)

  • 阳性对照
    • IHC-P: Mouse spleen FFPE tissue sections.
  • 常规说明


    ab25228 has been shown to block H2Ld-specific and H2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, but it does not block recognition of H2Ld-positive target cells by Ly-6G2-positive NK cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • Primary antibody说明ab25228 has been shown to block H2Ld-specific and H2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, but it does not block recognition of H2Ld-positive target cells by Ly-6G2-positive NK cells.
  • 克隆单克隆
  • 克隆编号28-14-8
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab25228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use a concentration of 2 µg/ml.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
  • 细胞定位Cell Membrane; single pass type I membrane protein
  • 数据库链接
  • 别名
    • H 2D cell surface glycoprotein antibody
    • H2 class I histocompatibility antigen, DB alpha chain antibody
    • H2D(B) antibody
    • H2D1 antibody
    • Histocompatibility 2, D region locus 1 antibody
    see all

Anti-MHC Class 1 H2 Db antibody [28-14-8] 图像

  • IHC image of MHC Class 1 H2 Db staining in mouse spleen formalin fixed paraffin embedded tissue section, using Mouse-on-Mouse detection kit, ab127055. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. The section was incubated with ab25228, 10µg/ml, for 15 mins at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

  • Flow Cytometry analysis of BALB/c mouse splenocytes labeling MHC Class 1 H2 Db with ab25228 at 1 μg/106 cells (purple). A Goat Anti-Mouse IgG2a, Human ads-FITC was used as the secondary antibody. Grey - Isotype Control, Mouse IgG2a-UNLB  followed by Goat Anti-Mouse IgG2a, Human ads-FITC.

  • Overlay histogram showing SP0/2 cells stained with ab25228 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25228, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-MHC Class 1 H2 Db antibody [28-14-8] (ab25228)参考文献

This product has been referenced in:
  • Kündig TM  et al. Fibroblasts as efficient antigen-presenting cells in lymphoid organs. Science 268:1343-7 (1995). Read more (PubMed: 7761853) »
  • Mason LH  et al. Cloning and functional characteristics of murine large granular lymphocyte-1: a member of the Ly-49 gene family (Ly-49G2) J Exp Med 182:293-303 (1995). Read more (PubMed: 7629495) »

See all 6 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"